Mice with experimental neuropsychiatric lupus (NPSLE), induced by anti-ribosomal-P antibodies, developed depression-like behavior and a diminished sense of smell. Manganese-enhanced MRI (MEMRI) allows in vivo mapping of functional neuronal connections in the brain, including the olfactory tract. The aim of this study was to analyze and describe, via the MEMRI technique, the effect of the anti-ribosomal-P injection on the olfactory pathway. Twenty mice were intra-cerebra-ventricular injected to the right hemisphere: 10 with human anti-ribosomal-P antibodies and 10 with human IgG antibodies (control). Depression was addressed by forced swimming test and smell function was evaluated by smelling different concentrations of menthol. MEMRI was used to investigate the olfactory system in these mice. Passive transfer of anti-ribosomal-P to mice resulted in a depression-like behavior, accompanied with a significant deficit in olfactory function. MEMRI of these mice demonstrated significant reduction (P < 0.001) in normalized manganese enhancement ratios of olfactory structures, compared to control mice. We concluded that an impaired olfactory neuronal function in mice with experimental depression, mediated by passive transfer of human-anti-ribosomal-P, can be demonstrated by MEMRI.
SUMMARYIgG antibodies to a 57-kD protein (p57) present in various human and bovine extracts were detected by immunoblotting in the serum of the mother of a baby with congenital heart block, but not in the corresponding cord blood, suggesting specific antibody consumption in the baby. Since this indicates a possible functional role for these antibodies, the antigen target was characterized and the association of the antibodies to heart block was further studied. A human K562 Agtl 1 cDNA library was screened and two clones were identified whose products reacted with the prototype serum. Antibody affinity-purified by use of the cloned gene products reacted on immunoblot with the 57-kD band. Partial sequences of both inserts were identical, but differed from DNA encoding the Ro(SSA) and La(SSB) antigens. Antibodies to the p57 were detected in 10% of systemic lupus erythematosus (SLE) sera, almost exclusively in association with antiRo(SSA). Furthermore, they were present in 38% (8/21) mothers of babies with neonatal lupus expressing either cardiac or cutaneous manifestations. Antibodies to this 57-kD protein may be an additional risk factor for neonatal lupus in anti-Ro-positive women. Moreover, disappearance of antibody from cord blood suggests that they may have a role in disease manifestations.
Nitrogen coiled tubing fracturing is the predominant method for completing and stimulating dry coalbed methane (CBM) formations, such as the Horseshoe Canyon in the Western Canadian Sedimentary basin. A typical well has an average of 20 pay zones that are stimulated individually. The coal cleats are fractured by pumping nitrogen at high rates through coiled tubing (CT) into perforations isolated by straddle assembly. Currently energy that can be delivered to the coalface of these dry CBM wells has been limited by the friction pressure through (CT). Efforts to increase the energy have involved increasing CT size and increasing surface horsepower. Economics and logistics practically limit the pipe size to 2 7/8 in. for deeper wells and 3¼ in. for shallow wells using CT fracturing technique. This paper discusses the development of a technique which initially eliminates the CT friction limits on transferring energy to coalface. A large-volume pressure pulse is released downhole during the fracturing process to create an order of magnitude change in available energy at the fracture face, compared to current dynamic fracturing processes. The additional energy is dissipated by increasing the surface cleat area exposed. The development of the technique and field tests results will be discussed. Introduction The Horseshoe canyon dry coal formations have been commercially exploited since late 2000. From the beginning it was clear the Horseshoe Canyon CBM play was a unique CBM play, it is set apart by consisting of a dry under pressured coal. The coal therefore does not require dewatering before production. The coal consists of multiple thin seams, ranging from 10 - 30 seams per well. The coal seam thickness ranges lies between 0.1 and 2.5 meters. (Fig 1 - Horseshoe Canyon CBM log) The production rates from these CBM wells dictates that a efficient drilling and completion model be adopted in order to be economic. The current model that is utilized is the wells are drilled with coiled tubing drilling rigs, cased and cemented. Wireline perforating crews are then dispatched to perforate the zones of interest in a rig less operation. The individual coal seams are then stimulated via the injection of dry nitrogen at high rates through a coiled tubing reel that is equipped with a fracturing isolation tool. The dry coal has not responded well to any other form of stimulation. The lack of success of more traditional stimulation methods is deemed to be damage to the dry under pressured coal seams.
PN mice spontaneously develop, with age, a lupus-like disease. The present study further evaluated autoantibody production in female PN mice. As early as 1 month of age, all PN mice had detectable IgM antibodies to dsDNA and ssDNA and two-thirds produced IgM anticardiolipin antibodies. By 3 months of age, all PN mice exhibited evidence of isotype switch in their autoantibody response; 88-100% had serum IgG antibodies to ssDNA and dsDNA, respectively. By 6-12 months of age, essentially all female PN mice had IgG antibodies to ssDNA, dsDNA, cardiolipin and other phospholipids (PS, PC, PI, and PG), and IgG and 63% produced IgG anti-mouse erythrocyte antibodies. In addition, 50-100% produced IgA antibodies to dsDNA and ssDNA, and one-third produced IgA anti-IgG antibodies. Antibodies to U1RNP and Sm were present in 81% of 6- to 12-month-old PN mice and 39-94% had IgG or IgM antibodies to mouse thymocytes. Although all four IgG isotypes were represented in the anti-dsDNA response, IgG1 antibodies dominated the IgG anticardiolipin response. The presence of IgA autoantibodies and the predominance of IgG1 in the IgG anticardiolipin response suggest that IL-4 and either IL-5 and/or TGF-beta serve as B cell stimulatory cytokines for autoreactive B cells in PN mice.
Exploration and exploitation of the shale formations in the United States has continued to accelerate since the early 2000's. Once considered to exist only as a cap rock, shale is now proving to be one of the most important reservoir rocks in North America and throughout the world. The demand for production logging (PL) in these reservoirs has increased due to the desire to better understand the basis behind higher stimulation efficiency and increased production results. Data collected via production logs can be used to evaluate efforts to enhance production through better zonal targeting, modifications to completion/hydraulic fracture design and/or alternate zonal isolation procedures. Drilling extended reach horizontal wells has allowed for more efficient exploitation of these formations but has also increased the number of potential obstacles to overcome in order to obtain complete and accurate production logs. Some of these obstacles include: severe doglegs which interfere with logging tool bending capability, substantial wellbore debris and/or added friction surface contact impacting depth of data capture, and completion configurations that limit equipment options and ease of logging. This paper will identify these critical factors and address proper candidate well selection and job preparation. It will also illustrate a multi-well logging campaign in the Marcellus shale, which highlights the benefits of fiber optic technology as a suitable alternative to traditional production logging techniques. .
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