The present study aims to valorize the Typha australis stems, through second-generation bioethanol production using enzymatic hydrolysis and fermentation. The monitoring of fermentation kinetics parameters, such as pH, density, length of fermentation, and the Brix, indicated a great variability of these parameters during the fermentation process of the must with three Saccharomyces cerevisiae strains, such as Angel brand Thermal-tolerant alcohol active dry yeast, Angel brand super alcohol active dry yeast and Angel super alcohol active dry yeast in the presence of urea (CON2H4) used as a growth factor. The distillation of musts after fermentation has yielded ethanol extraction rate (% v / v at 20 ° C) between 4.95and 44.93 after fractional distillation. The best performance in ethanolic bioconversion was recorded with Angel brand super alcohol active dry yeast. This Saccharomyces cerevisiae strains could be used as effective ferments, in perspective of intensive production of second-generation bioethanol with Typha australis stems.

This study aims to enhance agricultural resources and reduce post-harvest losses by assessing the physical and chemical characteristics of fruits and non-conventional vegetable oil extracted from Chrysophyllum albidum seeds in Benin. In this context, the fruits of Chrysophyllum albidum were collected in Tori (South Benin) and the morphological characteristics (mass, height and diameter at the equator) of the fruits, as well as the physico-chemical parameters (dry matter content, moisture content, ash content) were evaluated. The pH, soluble dry matter (Brix) and glucose levels of the juice were also evaluated. The extraction of vegetable oil from the seeds was carried out in Soxhlet followed by the determination of biochemical parameters such as quality indices such as acid, peroxide, saponification and iodine indices. The physicochemical characterisation of this oil was also carried out through the determination of density, refractive index and net calorific value (PCI). Analysis of these results reveals that the fruits of Chrysophyllumalbidum have a mean equator mass, size and diameter of 43.79±3.15g, 48.73±2.97mm and 41.11±1.13mm respectively. The levels of dry matter, moisture, and ash are respectively 51.60±0.47%, 48.40±0.47%, and 24.7±0.96% with a pH of 3.67±0.12 a Brix of 27.47±0.99 and a glucose level of 27.57mg/mL. The oil extraction efficiency is 21.52 ± 0.86% with a water and volatile matter content of 2.21 ± 0.64%. This vegetable oil has a dark red colour with an iodine value of 33.21 ± 0.08 g I2 /100 g-Oil, a saponification value of 188.40 ± 4.18 mg KOH / g-Oil, an acid value of 3.60 ± 0.23 mg KOH / g-Oil, a peroxide value of 10.46 ± 0.12 meq O2/g-Oil and an ester value of 155.19 ± 0.17 mg KOH / g-Oil. The density of vegetable oil at 30°C is 0.89. The refractive index of vegetable oil at 30°C is 1.48 with a lower calorific value of 40287.92 kJ/kg. The results thus obtained indicate possible ways of using Chrysophyllum albidum fruits as biofuel.

In the context of energy transition and the reduction of greenhouse gas emissions, the production of second-generation bioethanol is also recognized as a promising way to reduce our dependence on fossil fuels. Then, the present studies aim to evaluate the enzymatic biocatalysis of biomass from aquatic plant Phragmite karka in the second-generation bioethanol production. Results obtained revealed a rapid decrease of °Brix during the fermentation of musts and underlined the efficacy of enzyme hydrolysis. The rate of sugar consumption by yeasts is between 32.43 and 70.27%. The yield of ethanol production of yeasts indicated that Angel Brand Thermal-tolerant alcohol active dry yeast was the best yeast strain for this fermentation. These findings underline the potential of Phragmite karka plant materials in the perspective of intensive production of second-generation bioethanol.