The thin-layer chromatographic (TLC) behaviour of sma!: unilamellar liposomes containing inositol phosphates (IPS) was studied. The vesicles contained different conwntrations of D-myo-inositol 1,4,5-triphosphate (IPS), D myo-inositol 1,2,6-triphosphate (a-trinositol, PP 56, a novel Perstorp Pharma derivative), wmyo-inositol 1,3,4$-tetraphosphate (IP4), D-myo-inositol 1,3,4,5,6-pentakisphosphate (IP,) and mmyo-inositol lfJ,4$,6-hexakisphosphate (IPJ. Migration of all liposome batches was compared to that of control liposomes (multilamellar and small unilamellar, both containing only triple-distilled water), and to that of free phosphatidylcholine (PC).The same amount of lipid was used in all situations.Thin-layer chromatography was performed with silica gel as adsorbent. The developing solvent was an nbuthano1:ethanol:water mixture in a 4:3:3 volume ratio.At doses higher than lo-* M liposomes containing a-trinositol and IP, had a different migration than PC, MLV or SUV as well as all batches of liposomes. Physiological studies (using as model endothelized rat aorta rings) proved that in this situation they had no effects.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.