To investigate the role of jasmonate in the defense of plants against fungal pathogens, we have studied a mutant of Arabidopsis, fad3-2 fad7-2 fad8, that cannot accumulate jasmonate. Mutant plants were extremely susceptible to root rot caused by the fungal root pathogen Pythium mastophorum (Drechs.), even though neighboring wild-type plants were largely unaffected by this fungus. Application of exogenous methyl jasmonate substantially protected mutant plants, reducing the incidence of disease to a level close to that of wild-type controls. A similar treatment with methyl jasmonate did not protect the jasmonate-insensitive mutant coi1 from infection, showing that protective action of applied jasmonate against P. mastophorum was mediated by the induction of plant defense mechanisms rather than by a direct antifungal action. Transcripts of three jasmonate-responsive defense genes are induced by Pythium challenge in the wildtype but not in the jasmonate-deficient mutant. Pythium species are ubiquitous in soil and root habitats world-wide, but most (including P. mastophorum) are considered to be minor pathogens. Our results indicate that jasmonate is essential for plant defense against Pythium and, because of the high exposure of plant roots to Pythium inoculum in soil, may well be fundamental to survival of plants in nature. Our results further indicate that the fad3-2 fad7-2 fad8 mutant is an appropriate genetic model for studying the role of this important signaling molecule in pathogen defense.
The defining features of any cropping system are (i) the crop rotation and (ii) the kind or intensity of tillage. The trend worldwide starting in the late 20th century has been (i) to specialize competitively in the production of two, three, a single, or closely related crops such as different market classes of wheat and barley, and (ii) to use direct seeding, also known as no-till, to cut costs and save soil, time, and fuel. The availability of glyphosate-and insect-resistant varieties of soybeans, corn, cotton, and canola has helped greatly to address weed and insect pest pressures favored by direct seeding these crops. However, little has been done through genetics and breeding to address diseases caused by residue-and soil-inhabiting pathogens that remain major obstacles to wider adoption of these potentially more productive and sustainable systems. Instead, the gains have been due largely to innovations in management, including enhancement of root defense by antibiotic-producing rhizosphere-inhabiting bacteria inhibitory to root pathogens. Historically, new varieties have facilitated wider adoption of new management, and changes in management have facilitated wider adoption of new varieties. Although actual yields may be lower in direct-seed compared with conventional cropping systems, largely due to diseases, the yield potential is higher because of more available water and increases in soil organic matter. Achieving the full production potential of these more-sustainable cropping systems must now await the development of varieties adapted to or resistant to the hazards shown to account for the yield depressions associated with direct seeding.
The following are summaries of six of the papers presented at a Joint Meeting of the Scottish and East Anglia Regions and the Agricultural Group of the Industrial Division held on June 29th-30th, 1987, in the University of Aberdeen.
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