Five polyenoic acids present in human testicular tissue have been isolated in pure form by gas chromatography and chemically identified using procedures of hydrogenation and UV absorption spectrometry following alkaline isomerization and ozonolysis. Three of these (20∶3, 22∶4 and 22∶5) belong to the linoleic acid and the other two (22∶6 and 22∶5), to the α‐linolenic acid family. The latter (22∶5ω3) had not been reported previously in human testes; the others had been tentatively identified by retention time using gas chromatography.
W-H NORMAL L -V V 4 >-Q 200 n 3 160 w 120 2 80 I -0 5 10 15 20 25 t P FIG. 1 . Trypsin, invertase and amylase content of feces of germ free, ex-germfree and conventional rats.,4t P germfree animals were infected with sporeforming bacteria from the cecum of a conventional animal and at TF with suspension of feces from conventional rats.Amylase content of the feces in the germfree rats was the same or slightly higher than that of the conventional rats. The occurrence of invertase in the feces of germfree animals agrees with the finding of carbohydrases in intestinal extracts from germfree rats(8).Allcr infection of the g,rcrmfrw aniinals with \powforining hacxteria from the cecum content of a convcntiorial animal, tio decrease occurred in the enzymatic contents of the feces but rather sharp fluctuations were noticed in invertase and trypsin activity. This might be connected with the diarrhea that is a constant symptom in infection of germfree animals with certain intestinal bacteria. After superinfection with full intestinal flora of the conventional animals, tryspin content of the feces decreased to the zero values of the conventional rats within 24 hours.Conclusions. Results indicate that autodigestion is not the main pathway for normally occurring inactivation of trypsin and invertase. The results infer that one or several of the normal microbiological inhabitants of the intestines are responsible for normally occurring inactivation of the digestive enzymes of intestinal contents.
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