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The carbon use efficiency (CUE) of soil microorganisms is a critical parameter for the first step of organic carbon (C) transformation by and incorporation into microbial biomass and shapes C cycling in terrestrial ecosystems. As C and nitrogen (N) cycles interact closely and N availability affects microbial metabolism, N addition to soil may shift the microbial CUE. We conducted a meta‐analysis (100 data pairs) to generalize information about the microbial CUE response to N addition in soil based on the two most common CUE estimation approaches: (i) 13C‐labelled substrate addition (13C‐substrate) and (ii) 18O‐labelled water addition (18O‐H2O). The mean microbial CUE in soils across all biomes and approaches was 0.37. The effects of N addition on CUE, however, were depended on the approach: CUE decreased by 12% if measured by the 13C‐substrate approach, while CUE increased by 11% if measured by the 18O‐H2O approach. These differences in the microbial CUE response depending on the estimation approach are explained by the divergent reactions of microbial growth to N addition: N addition decreases the 13C incorporation into microbial biomass (this parameter is in the numerator by CUE calculation based on the 13C‐substrate approach). In contrast, N addition slightly increases (although statistically insignificant) the microbial growth rate (in the numerator of the CUE calculation when assessed by the 18O‐H2O approach), significantly raising the CUE. We explained these N addition effects based on CUE regulation mechanisms at the metabolic, cell, community, and ecosystem levels. Consequently, the differences in the microbial responses (microbial growth, respiration, C incorporation, community composition, and dormant or active states) between the 13C‐substrate and 18O‐H2O approaches need to be considered. Thus, these two CUE estimation approaches should be compared to understand microbially mediated C and nutrient dynamics under increasing anthropogenic N input and other global change effects.

Soil respiration is the second largest terrestrial carbon (C) flux; the responses of soil respiration to nitrogen (N) deposition have far-reaching influences on the global C cycle. N deposition has been documented to significantly affect soil respiration, but the results are conflicting. The response of soil respiration to N deposition gradients remains unclear, especially in ecosystems receiving increasing ambient N depositions. A field experiment was conducted in a natural evergreen broadleaf forest in western China from November 2013 to November 2015 to understand the effects of increasing N deposition on soil respiration. Four levels of N deposition were investigated: control (Ctr, without N added), low N (L, 50 kg N ha−1·a−1), medium N (M, 150 kg N ha−1·a−1), and high N (H, 300 kg N ha−1·a−1). The results show that (1) the mean soil respiration rates in the L, M, and H treatments were 9.13%, 15.8% (P < 0.05) and 22.57% (P < 0.05) lower than that in the Ctr treatment (1.56 ± 0.13 μmol·m−2·s−1), respectively; (2) soil respiration rates showed significant positive exponential and linear relationships with soil temperature and moisture (P < 0.01), respectively. Soil temperature is more important than soil moisture in controlling the soil respiration rate; (3) the Ctr, L, M, and H treatments yielded Q10 values of 2.98, 2.78, 2.65, and 2.63, respectively. N deposition decreased the temperature sensitivity of soil respiration; (4) simulated N deposition also significantly decreased the microbial biomass C and N, fine root biomass, pH and extractable dissolved organic C (P < 0.05). Overall, the results suggest that soil respiration declines in response to N deposition. The decrease in soil respiration caused by simulated N deposition may occur through decreasing the microbial biomass C and N, fine root biomass, pH and extractable dissolved organic C. Ongoing N deposition may have significant impacts on C cycles and increase C sequestration with the increase in global temperature in evergreen broadleaf forests.