No abstract

The autolytic enzyme (endo-,B-1,4-N-acetylmuramoylhydrolase) of Streptococcus faecium (S. faecalis ATCC 9790) was released in a soluble form from insoluble cell wall-autolytic enzyme complexes by treatment with dilute NaOH at 00G; Treatment of cell wall-enzyme complexes, obtained from either exponentialor stationary-phase cells, with 0.008 to 0.01 N NaOH gave maximum yields of autolytic enzyme activity. At a fixed concentration of NaOH, the yield of autolysin increased with increasing wall densities and was accompanied by the release of methylpentose and phosphorus in amounts proportional to the autolysin. Since source of enzyme, alkaline extracts have major advantages over wall lysates and LiCl extracts, 699 on August 5, 2020 by guest

No abstract

No abstract