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The crystal structure of the form I of syndiotactic poly(1-butene) is presented. According to the present analysis, chains having s(2/1)2 helical conformation are packed in an orthorhombic unit cell with a = 16,81 A, b = 6,06 A and c = 7,73 A . The density is 0,96 g/cm3 with two chains in the cell (8 monomeric units), the space group is C222,. Disorder, corresponding to the statistical presence of right-and left-handed helices in each site of the lattice, could be present. The space group for the completely disordered structure is Cmcm.

SUMMARY An association between increased blood pressure and hypalgesia has been reported in several studies in animals and in a few reports in humans. We investigated the relationship between hypertension and pain perception by comparing the response to graded electrical stimulation of the tooth pulp, which is thought to represent an exclusively nodceptive system. The test was performed with a commercial tooth pulp tester in a large series of subjects with borderline or established hypertension and in three groups of normotensive controls: volunteers, nonhypertensive patients, and medical students with a well-established or no family history of hypertension. Subjects had to report when they started to feel pulp stimulation (sensory threshold) and when this became painful (pain threshold). Sensory and pain thresholds were obtained as means of the measurements on four healthy, unfilled teeth. Sensory thresholds were significantly higher hi subjects with borderline or established hypertension than in two of the three normotensive groups (volunteers and normotensive patients), whereas no significant difference was observed between the two hypertensive groups. The results for the pain threshold were qualitatively similar but less dear and less amenable to statistical analysis because this parameter could not be determined with accuracy hi a number of subjects in whom the subjective pain threshold was above the upper range of stimulation of the instrument. The association between Mood pressure levels and pain perception was further confirmed by the highly significant correlation found for the overall data between mean arterial blood pressure and both thresholds. On the other hand, no significant correlation was found with heart rate and no significant effects could be detected by analysis of variance for sex, age, and family history of hypertension. Furthermore, no changes hi pain sensitivity were observed hi a subgroup of patients studied after 3 months of diuretic or ^-blocking treatment or of low salt diet, despite significant reductions of arterial blood pressure. Taken together, our findings provide further confirmatory evidence for an increased tolerance to pain in hypertensive humans and suggest that this may be a feature of arterial hypertension irrespective of the prevailing blood pressure levels. Psychophysiological studies in the rat 2 -9 have demonstrated the association of hypalgesic behavior (delayed response to noxious stimuli such as a hot plate, an electric shock, or a mechanical force applied to a limb) to arterial hypertension. In hypertensive humans, an increased tolerance to pain, as assessed by the measurement of the pain threshold to graded electrical tooth pulp stimulation, has been reported by Zamir and Shuber 10 and confirmed in preliminary studies by our group."•

PRDM (PRDI-BF1 and RIZ homology domain containing) protein family members are characterized by the presence of a PR domain and a variable number of Zn-finger repeats. Experimental evidence has shown that the PRDM proteins play an important role in gene expression regulation, modifying the chromatin structure either directly, through the intrinsic methyltransferase activity, or indirectly through the recruitment of chromatin remodeling complexes. PRDM proteins have a dual action: they mediate the effect induced by different cell signals like steroid hormones and control the expression of growth factors. PRDM proteins therefore have a pivotal role in the transduction of signals that control cell proliferation and differentiation and consequently neoplastic transformation. In this review, we describe pathways in which PRDM proteins are involved and the molecular mechanism of their transcriptional regulation.

Samples of isotactic polypropylene in the α form may have different degrees of order in the up and down positioning of the chains. By suitable programming of the crystallization history of unoriented samples it is possible to show, from DSC and WAXS data, that a strict correlation exists between the melting behavior and the degree of ordering of the chains. The process of ordering at annealing temperatures above 150°C may lead to a “continuum” of modifications of the α form from less ordered to highly ordered ones; correspondingly we observe an increase in the melting point by as much as 15°C.

We report that in breast cancer cells, tyrosine phosphorylation of the estradiol receptor alpha (ERalpha) by Src regulates cytoplasmic localization of the receptor and DNA synthesis. Inhibition of Src or use of a peptide mimicking the ERalpha p-Tyr537 sequence abolishes ERalpha tyrosine phosphorylation and traps the receptor in nuclei of estradiol-treated MCF-7 cells. An ERalpha mutant carrying a mutation of Tyr537 to phenylalanine (ER537F) persistently localizes in nuclei of various cell types. In contrast with ERalpha wt, ER537F does not associate with Ran and its interaction with Crm1 is insensitive to estradiol. Thus, independently of estradiol, ER537F is retained in nuclei, where it entangles FKHR-driving cell cycle arrest. Chromatin immunoprecipitation analysis reveals that overexpression of ER537F in breast cancer cells enhances FKHR interaction with cyclin D1 promoter. This mutant also counteracts cell transformation by the activated forms of Src or PI3-K. In conclusion, in addition to regulating receptor localization, ERalpha phosphorylation by Src is required for hormone responsiveness of DNA synthesis in breast cancer cells.

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