Here we report on the complete cDNA sequence of the b-subunit of human factor xI (FXIII). FXII is a blood coagulation protein that is distributed both extracellulary and intracellulary (1). Its enzymatic form acts as a transglutaminase and is also called activated fibrin-stabilizing factor or fibrinoligase (EC 2.3.2.13, protein-glutamine:amine gamma-glutamyltransferase). In blood plasma FXII was described to consist of two catalytic a-subunits and in addition two b-subunits (together MW 320 000).A full length cDNA clone encoding the FXIIIb subunit was isolated from a human liver lambda gtlO library by screening with oligonucleotides generated from the FXIIb encoding cDNA sequence published by Ichinose et al. (2). These authors presented a FXIIIb cDNA sequence lacking the 5'-end of the mRNA and the translational start codon. Our 2613 bp cDNA comprises 420 bp of the 5'-untranslated region (including EcoRI linkers) and encodes the total protein with 661 residues (MW 75 491) including the complete hydrophobic signal sequence (residue -20 to -1).
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