Background Gastric cancer is the third leading cause of cancer-related deaths worldwide and has been associated with infections that may promote tumour progression. Accordingly, we analysed the presence of Mollicutes, Mycoplasma hyorhinis, Fusobacterium nucleatum and Helicobacter pylori in gastric cancer tissues and evaluated their correlation with clinicopathological factors. Methods Using a commercial kit, DNA were extracted from 120 gastric samples embedded in paraffin: 80 from patients with gastric cancer and 40 from cancer free patients, dating from 2006 to 2016. Mollicutes and H. pylori were detected by PCR; F. nucleatum and M. hyorhinis were detected by qPCR, together with immunohistochemistry for the latter bacteria. Results Mollicutes were detected in the case and control groups (12% and 2.5%) and correlated with the papillary histologic pattern (P = 0.003), likely due to cell transformation promoted by Mollicutes. M. hyorhinis was detected in the case and control group but was not considered a cancer risk factor. H. pylori was detected at higher loads in the case compared to the control group (8% and 22%, P = 0.008) and correlated with metastasis (P = 0.024), lymphatic invasion (P = 0.033), tumour of diffused type (P = 0.028), and histopathological grading G1/G2 (P = 0.008). F. nucleatum was the most abundant bacteria in the case group, but was also detected in the control group (26% and 2.5%). It increased the cancer risk factor (P = 0.045, OR = 10.562, CI95% = 1.057–105.521), and correlated with old age (P = 0.030) and tumour size (P = 0.053). Bacterial abundance was significantly different between groups (P = 0.001). Conclusion Our findings could improve the control and promote our understanding of opportunistic bacteria and their relevance to malignant phenotypes.
Tobacco use and alcohol consumption are the principal risk factors implicated in head and neck cancers, however, the presence of HPV has also been associated. Here, we sought to correlate risk factors such as socio-demographic and behavioral variables, and the presence of HPV, to head and neck cancer occurrence. During August 2016 – December 2017, paraffin embedded samples from two anatomic pathology services of two populous cities in the state of Bahia were analyzed. To detect the presence of HPV, the formalin-fixed, paraffin embedded tissue samples were initially deparaffinized for subsequent DNA extraction. Nested-PCR was applied to detect HPV DNA, and viral subtyping was confirmed through specific PCR primer and sequencing. Most of the patients confirmed being smokers and drinkers. HPV was detected only in 7% of the samples, a histopathological diagnosed benign lesion of laryngeal papilloma (HPV 11), and a malignant lesion of the hard palate (HPV type not specified). Our findings indicated that tobacco use and alcohol consumption were correlated as the highest risk factors for the development of neoplasms. Although HPV prevalence was low, we could not neglect HPV involvement in head and neck cancers in individuals from Bahia State. Furthermore, HPV+ cancers respond better to therapy, therefore, defining the type of tumor is important to determine the most effective treatment.
A incidência de neoplasias de mama, estômago e próstata têm aumentado consideravelmente no mundo, decorrente das mudanças no perfil de morbidade e mortalidade. Estudos acerca da ocorrência deste tipo de patologia fornecem informações sobre o perfil epidemiológico de determinada região, o que reflete diretamente a importância do estudo no controle e prevenção da doença, além do desenvolvimento de medidas para tratamento. Deste modo, o objetivo deste estudo foi analisar a ocorrência de neoplasias de mama, de próstata e de estômago, bem como caracterizar seu perfil histopatológico, na microrregião de Vitória da Conquista, Bahia. Foram analisados 3269 pacientes, correspondentes ao período de 2009 a 2013, disponibilizados pelo Laboratório de Anatomia Patológica e Citopatologia MICRO, através da aplicação de um questionário para análise de variáveis como gênero, idade, ano da biópsia, neoplasia diagnosticada e seu subtipo histológico. Dentre as neoplasias investigadas, próstata mostrou-se mais ocorrente, correspondendo a 70,5% dos laudos analisados. Seguido de mama, que apresentou como subtipo histológico mais frequente o carcinoma ductal infiltrante. O adenocarcinoma gástrico do tipo intestinal foi o tipo histológico predominante encontrado na coleta dentre as neoplasias do estômago. Deste modo, o estudo fornece uma base acerca do perfil de ocorrência das neoplasias na microrregião de Vitória da Conquista, indicando que estas seguem os padrões epidemiólogicos relatados anteriormente para outras regiões.Palavras-chave: neoplasias gástricas; neoplasias da próstata; carcinoma ductal.The incidence of breast cancer, stomach and prostate cancers have increased considerably in the world, resulting from changes in the morbidity and mortality profile. Studies on the occurrence of this type of pathology provide information on the epidemiological profile of a given region, which directly reflects the importance of studying the control and prevention of disease, and the development of measures for treatment. Thus, the aim of this study was to analyze the occurrence of breast, prostate and stomach cancers as well as your histological profile in the microregion of Vitória da Conquista, Bahia. To do so, 3269 patients were analyzed, for the period 2009-2013, provided by the Laboratory of Pathology and Cytopathology MICRO, by applying a questionnaire to analyze variables such as gender, age, year of biopsy and its histological subtype. Among the investigated cancer, prostate was more occurring, corresponding to 70.5% of the analyzed reports. Followed by breast neoplasias, who presented the most common histological subtype infiltrating ductal carcinoma. Gastric adenocarcinoma intestinal type was the predominant histologic type found in the collection among the cancers of the stomach. Thus, the study provides a basis about the occurrence profile of cancers in the microregion of Vitória da Conquista, indicating that they follow the previously reported epidemiological patterns to other regions.
Human cysticercosis is a neglected tropical parasitic disease due to the tapeworm Taenia solium, common in endemic developing countries. Cysticerci are most commonly found within the central nervous system, but they may also localize in a variety of tissues, including the tongue. Here, we described a case of a 21-year-old woman with a painless, firm, smooth, mucosa-colored nodule located in the lateral region of the tongue was seen by a dentist. An excisional biopsy was performed, and the surgical specimen was examined histomorphologically. The analysis revealed the presence of a cystic lesion containing a serrated larva (Cysticercus cellulosae) as well as a cystic capsule with predominantly mononuclear inflammation. The morphological findings were consistent with the diagnosis of cysticercosis of the tongue. The patient was followed for 22 months and showed no signs of recurrence.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.