Sugar cane silage has a potential for animal feeding, but uncontrolled growth of undesirable microorganisms may cause nutritional losses and affect the animal productivity and health. The objective of this work was to evaluate the microbiological quality and chemical composition of ensiled sugar cane with and without nutritional additives after 30 days of fermentation. Yeasts, filamentous fungi and distinct groups of bacteria were enumerated by plate count methods and the chemical analyzes comprised dry matter, crude protein, fiber content, lignin, and pH. Facultative aerobic bacteria and filamentous fungi were not detected during the fermentative process in any of the treatments. The number of yeasts in five varieties of sugar cane silage without additives was about 6.55 Log CFU g -1 of silage, and with 1% ammonium sulfate and 1% urea were about 5.86 and 5.50 Log CFU g -1 of silage, respectively. The lactic acid bacteria (LAB) count without additive was about 8.62 Log CFU g -1 of silage, and with 1% ammonium sulfate and 1% urea the count was about 6.40 and 6.54 Log CFU g -1 of silage, respectively. The average percent of dry material in the three treatments was 20.76%. The addition of ammonium sulphate and urea has decreased the microbial load after 30 days but it has increased the total crude protein concentration. Additives also affected neutral detergent fiber, acid detergent fiber and lignin content in all five varieties of sugar cane silage.

Relato de Caso www.higieneanimal.ufc.br Diagnóstico do conhecimento das práticas higiênico-sanitárias nas operações de ordenha em propriedades rurais produtoras de leite de cabra Diagnostic of knowledge of practice hygienic-sanitary in operations by milking in rural properties produce of milk from goat.

The present paper studies the influence of different nutrients for the production of two cellulolytic enzymes: endo beta-1.4 glucanase and exo beta-1.4 glucanase by anaerobic fungi taken from cow rumen, that were fed a diet of corn silage and Brachiaria decumbens grass hay. During the enzymatic degradation assays, it was observed that the addition of some essential nutrients in the formulation of the culture medium contributed positively in the cellulolytic enzyme production, with exception of riboflavin. Such results contributed in the establishment of an effective method for the evaluation of enzymatic activities in anaerobic fibrolytic fungi. In this work, nutrients added to enrich the culture medium have successfully proven that they can be used as inoculating agents (inductors) in diets rich in ensilage with law nutritive value.