ResumenLas plantas pertenecientes al género Solanum son conocidas por su amplio espectro de actividad biológica. Por esto, el objetivo de este trabajo fue evaluar la acción antibacteriana de extractos etanólicos y metanólicos de Solanum dolichosepalum sobre las cepas bacterianas Staphylococcus aureus, Salmonella spp., Pseudomonas aeruginosa y Aeromonas hydrophila. Los extractos fueron obtenidos por extracción sólido-líquido en equipos Soxhlet, con posterior concentración por evaporación rotatoria. Para determinar la actividad antibacteriana se usó el método de difusión en disco, empleando agar Mueller Hinton, Cloranfenicol (sensidiscos de 30 mg) como control positivo, y los solventes de extracción como controles negativos. Los extractos metanólicos y etanólicos de S. dolichosepalum mostraron un leve efecto inhibitorio contra S. aureus, Salmonella spp., A. hydrophila y P. aeruginosa, pero no fue suficiente para considerarse significativo mostrando resistencia a los mismos. Para los dos tipos de extractos usados, el etanólico fue el más activo sobre S. aureus, Salmonella spp., A. hydrophila, y el metanólico frente a P. aeruginosa. AbstractThe plants belonging to the genus Solanum are known for their broad spectrum of biological activity. Therefore, the objective of this work was to evaluate the antibacterial activity of ethanolic and methanolic extracts of S. dolichosepalum against bacterial strains S. aureus, Salmonella spp, P. aeruginosa, and A. hydrophila. The methanolic and ethanolic extracts were obtained by solid-liquid extraction in soxhlet equipment, with subsequent concentration by rotary evaporation. To determine the antibacterial activity, the disc diffusion method was used, using Mueller Hinton agar, Chloramphenicol (30 mg sensitives) as a positive control, and extraction solvents as negative controls. The methanolic and ethanolic extracts of S. dolichosepalum showed a slight inhibitory effect against S. aureus, Salmonella spp, A. hydrophila, and P. aeruginosa, but it was not enough to be considered significant showing resistance to them. The two types of extracts used, ethanolic was the most active against S. aureus, Salmonella spp, A. hydrophila, and methanolic against P. aeruginosa.
ResumenListeria spp. es un género bacteriano que contamina alimentos de origen animal, incluido el pollo, yl puede permanecer viable durante las cadenas de producción y distribución. En el presente trabajo se analizaron 91 muestras de carcasas de pollo, obtenidas en una distribuidora en el nororiente de Bogotá; las muestras se tomaron en un período de 9 semanas y luego fueron procesadas. De las 91 carcasas, 40 (43.95%) resultaron positivas para Listeria spp. La presencia de este microorganismo puede estar asociada a deficiencias en los sistemas de tecnología de limpieza en las plantas de beneficio y/o contaminación con utensilios durante el desprese.Palabras clave: biopelícula, carcasas de pollo, industria cárnica, Listeria spp., microorganismos psicrótrofos, Pseudomonas. Abstract Incidence of Listeria spp., in frozen poultry carcasses at supermarket in northwest BogotáListeria spp. it is a bacterial gene that contaminates foods of animal origin including poultry, where it can remain viable during the chains of production and distribution. In this work, 91 samples of chicken carcasses were analyzed; they were obtained in a distributor in northwest Bogota. The samples were taken in a period of 9 weeks and processed shortly thereafter. From the 91 carcasses, 40 (43.95%) were positive for Listeria spp. The presence of this microorganism can be associated to deficiencies in the systems of cleaning technology in the poultry factory and/or contamination with utensils during cut-up.
The lower basin of the Cusiana River is an area affected by various socioeconomic activities. This study aims to establish the physicochemical and biological structure of areas influenced by agriculture, livestock, and wastewater discharges from urban areas. Sampling was conducted at two locations, the municipality of Aguazul and the municipality of Maní in the Casanare department, Colombia. Four monitoring surveys were carried out considering seasonal differences in rainfall and drought. The variables analyzed were: temperature, alkalinity, conductivity, turbidity, BOD, COD, total hardness, total phosphorus, nitrates, dissolved oxygen, pH, suspended solids and sampling of benthic macroinvertebrate communities. To assess water quality in the sampled periods, physicochemical indicators ICA NSF were estimated, considering the intended uses of the water resource, and reporting average quality. A total of 1491 individuals were reported, distributed across seven orders and 20 families. The BMWP/Col index was calculated for the two monitoring stations. The Aguazul station showed doubtful water, categorized as slightly polluted waters, which decreased significantly to moderately polluted waters. The Maní Station transitioned from dubious to critical condition and from moderately polluted to very polluted waters. Studies of this nature allow entities to make informed decisions regarding the proper using and management of the region’s water systems.
<p><strong>Background. </strong>The study and monitoring of water quality is commonly carried out through the characterization of physicochemical and bacteriological parameters. However, the presence of point pollutants that are discharged at times when an exact record of their incidence may not be achieved, has led to the exploration of different methodologies in order to determine the quality of a body of water, one of which is the bioindication through the study of aquatic organisms. <strong>Objective.</strong> Analyze the biological quality of the Salitre river basin, Boyacá (Colombia) in areas of influence of mining and agricultural activity in the rainy season (April-May 2019) and dry season (September-October 2019). <strong>Methodology. </strong>The study was conducted by determining the diversity of aquatic macroinvertebrates present in three stations distributed in the upper (P1), middle (P2) and lower (P3) part of the river. <strong>Results. </strong>3573 individuals distributed in eight orders, 28 families and 39 genera were observed, standing out in wealth and abundance: Diptera (79.54%), Ephemeroptera (9.51%) and Odonata (6.07%). <strong>Implications. </strong>The main limitation of this work was the transfer of the water samples to the laboratory for the physicochemical analysis, for the maintenance of the cold chain during all the samplings. <strong>Conclusions.</strong> Changes were registered in physicochemical and biological conditions of the system, mainly for the diversity in the three sampling points, which represents a low capacity of this system to take better and better use of nutrients and energy in areas of anthropogenic intervention, affecting self-purification of the river and the biological activities of the ecosystem.</p>
Introducción: el agua apta para el consumo humano es aquella que no representa un riesgo para la salud del consumidor teniendo en cuenta sus características organolépticas, físicas, químicas y bacteriológicas, pero dicha calidad es afectada por vertimientos de actividades domésticos, industriales y económicas (Gamboa et al., 2015). Objetivo: este estudio se hizo con el fin de determinar la calidad microbiológica del río Toca, sector Tuaneca abajo y el Centro, Departamento de Boyacá, Colombia. Materiales y métodos: en la cuenca, se establecieron cuatro puntos de muestreo, M1, M2, M3 y M4, en cada punto se colectaron muestras de 50 mL de agua con 20 réplicas, en frascos de vidrio estériles, las cuales fueron refrigeradas a 4°C en neveras de icopor y procesadas en el menor tiempo posible en el laboratorio de Microbiología de la UPTC; en donde se realizó cuantificación de mesófilos aerobios, mohos y levaduras mediante recuento en placa; coliformes totales y fecales se evaluaron mediante la técnica de Número más Probable (NMP). Resultados: el punto de muestreo M1 presentó los mayores valores de coliformes totales con un valor de 1100 NMP/100mL, coliformes fecales: 43 NMP/100mL; así como de mohos y levaduras 61X103 UFC/mL; y el punto con mayor valor de mesófilos aerobios fue 13X104 UFC/ mL que corresponde al M2. Los puntos M3 y M4 presentaron ausencia de coliformes totales. Conclusiones: en contraste con el Decreto 1594 de 1984, sobre usos del agua y residuos líquidos, el río Toca presenta mala calidad de agua en los puntos de muestreo M1 y M2, los cuales presentan actividad ganadera y vertimientos de aguas residuales domésticas.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.