There exists a growing demand and promising market for new lettuce varieties in Brazil. Cv. Brunella is an innovative lettuce variety which mixes crisp lettuce and head lettuce characteristics besides being adapted to the Brazilian growing conditions. The physicochemical and sensory quality of this lettuce, cultivated under different growing systems, was evaluated. The conventional planting and the hydroponic systems were carried out in the experimental area of the Universidade Federal de São Carlos, São Paulo State, Brazil and the organic system was done by certified organic producer in the municipality of Cordeirópolis. After harvesting, leaf area, leaf size, unit leaf area, fresh mass, mass loss, turgor pressure, instrumental color, pH, total titratable acidity (TTA), total soluble solids (TSS), total phenolic compounds (TPC), and sensory characterization were evaluated. We verified that the growing system influenced on physicochemical traits producing lettuces of different sizes, weights and stability. For pH, TSS and TPC, difference among the three growing systems was not observed. For TTA, the hydroponic sample showed higher acidity. About sensory evaluation, the testers noticed difference for green color, thickness, size and leaf crispness, grass aroma and bitter taste. The samples cultivated in conventional and hydroponic systems showed greater preference and purchase intention.