Introduction: This study aimed to investigate the effects of photobiomodulation (PBM) therapy associated with biphasic calcium phosphate on calvaria critical defects in rats. Methods: Forty-eight (90 days old) adult male rats (Rattus norvegicus, Albinus variation, Wistar) received critical defects of 5 mm in diameter, which were made on their skull, and they were randomly assigned into the following groups: C-blood clot, B-biphasic calcium phosphate, L-photobiomodulation therapy, and B + L-biphasic calcium phosphate + photobiomodulation therapy. A low-level a gallium aluminum arsenide (GaAlAs) laser was applied in a single dose during surgery, in a wavelength of 660 nm and total energy density of 45 J/cm2. On 30th and 60th days, the animals from each group were euthanized. Histological and histomorphometric analyses were performed. Results: In 30 days, almost all specimens (C, L, B and B + L) showed bone neoformation areas in regions near the borders of the surgical defect. In 60 days, in many specimens (C, L, B, B + L), it was possible to see a narrow neoformed bone structure along almost the whole extension of the surgical defect, though it was thinner than the original calvary bone. Data were recorded as mean ± standard deviation, and after normality was tested, a suitable statistical test was applied (α = 5%). On day 60, there was a statistically significant difference when comparing the proportion of neoformation area between group L (0.52% ± 0.13) and group B + L (0.20% ± 0.08). Group L showed a difference compared with all the groups when we compared the remaining distance between the edges of neoformed bone (C × L, P = 0.0431; B × L, P = 0.0386; L × B + L, P = 0.0352), demonstrating a great defect closure. Conclusion: Our findings suggest that although biphasic calcium phosphate exerts some osteogenic activity during bone repair, PBM therapy is not able to modulate this process.
This study aimed to evaluate the Carbon Fiber obtained from PAN textile and cotton fiber in their different forms of presentation: non‐activated carbon fiber felt (NACFF), activated carbon fiber felt (ACFF), silver activated carbon fiber felt (Ag‐ACFF), and activated carbon fiber tissue (ACFT), to obtain scaffolds as a potential material with properties related to the synthetic bone graft. Characterization tests performed: surface wettability, traction, swelling, and in vivo tests: evaluation of the inflammatory response by implanting the materials in the subcutaneous tissue of 14 Wistar rats, evaluation of collagen fibers by picrosirius red staining and assessment of toxicity in the following organs: heart, spleen, liver, and kidney. In the wettability test, NACFF and ACFT were hydrophobic (θ124° and 114°), ACFF and Ag‐ACFF were hydrophilic. For maximum stress, ACFF was more resistant (2.983 ± 1.059) p < .05. In the swelling test, the Ag‐ACFF and ACFF groups showed the highest absorption percentage for the PBS solution and distilled water (p < .001). The organs showed no signs of acute systemic toxicity. The implant regions showed mild to moderate inflammatory infiltrate at 7 and 21 days. Only the ACFT group did not show the maturation of type I collagen fibers in 21 days. Through the conducted analyses, the ACFT shows little potential to be indicated as a possible scaffold. Therefore NACFF, ACFF, and Ag‐ACFF have the potential to be considered scaffolds due to the following characteristics presented: good absorption rate, hydrophilicity, and non‐toxic.
A Desordem temporomandibular (DTM) é uma condição em que há um comprometimento da articulação temporomandibular e/ou das estruturas adjacentes. Na maioria dos casos, seus sintomas são discretos e cíclicos, podendo ter variações no grau da limitação de abertura da boca e da dor, mas esta disfunção, pode também proporcionar um grande desconforto tornando-se uma doença gravemente incapacitante. Para a solução de problemas de desordem na articulação temporomandibular existem várias opções cirúrgicas, dentre elas a artroscopia. Considerada como uma técnica segura, ela alivia a dor severa de pacientes e restabelece a amplitude dos movimentos mandibulares. A artroscopia não é considerada uma técnica invasiva, portanto os pacientes possuem uma rápida recuperação. Nos últimos anos, muito se evoluiu nessa técnica, principalmente devido à melhor qualidade das imagens de ressonância magnética (IRM), possibilitando um melhor diagnostico e tratamento mais rápido.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.