CALCIUM PARTITION IN SERUM
647the test animals were found to give positive tuberculin tests after intervals of 4 and 8 weeks. Recovery of acid-fast organisms was made from the liver, spleen and lymph nodes.3. The cultivation of the organisms on Sweaney's egg-glycerol medium.Three separate series of tests were made on each type of membrane. Two guinea pigs were used for the controls and 2 for each test. Each animal was previously found to be tuberculosis-free as determined by the tuberculin test. An even emulsion of the organism was obtained by thoroughly grinding the growth as washed from the solid media in physiological saline.Our results show that the ultrafiltrates did not contain infectious particles capable of producing: (1) a positive tuberculin reaction;( 2 ) anatomical evidences of the infection in experimental animals or growth of tubercle bacilli on appropriate media for their cultivation. It may be concluded, therefore, that the cultures tested did not develop "filterable forms" smaller than 40 pp in diameter.It has now been thoroughly shown that under certain conditions the inorganic phosphate of the serum becomes incompletely diff usible.' A logical explanation of this phenomenon is that the nondiffusible phosphate which is formed is combined with some of the serum calcium in a compound which is in a colloidal state. As yet, no direct evidence is available as to the exact composition of this colloidal calcium phosphate.On the basis of the view which has recently received strong support from the work of McLean and Hastings,2 namely, that there is an equilibrium between the ionized and the protein bound calcium in
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