A line of sight toward the Galactic Center (GC) offers the largest number of potentially habitable systems of any direction in the sky. The Breakthrough Listen program is undertaking the most sensitive and deepest targeted SETI surveys toward the GC. Here, we outline our observing strategies with Robert C. Byrd Green Bank Telescope (GBT) and Parkes telescope to conduct 600 hr of deep observations across 0.7–93 GHz. We report preliminary results from our survey for extraterrestrial intelligence (ETI) beacons across 1–8 GHz with 7.0 and 11.2 hr of observations with Parkes and GBT, respectively. With our narrowband drifting signal search, we were able to place meaningful constraints on ETI transmitters across 1–4 GHz and 3.9–8 GHz with EIRP limits of ≥4 × 1018 W among 60 million stars and ≥5 × 1017 W among half a million stars, respectively. For the first time, we were able to constrain the existence of artificially dispersed transient signals across 3.9–8 GHz with EIRP ≥1 × 1014 W/Hz with a repetition period ≤4.3 hr. We also searched our 11.2 hr of deep observations of the GC and its surrounding region for Fast Radio Burst–like magnetars with the DM up to 5000 pc cm−3 with maximum pulse widths up to 90 ms at 6 GHz. We detected several hundred transient bursts from SGR J1745−2900, but did not detect any new transient bursts with the peak luminosity limit across our observed band of ≥1031 erg s−1 and burst rate of ≥0.23 burst hr−1. These limits are comparable to bright transient emission seen from other Galactic radio-loud magnetars, constraining their presence at the GC.

The paralogous multifunctional adaptor proteins YAP and TAZ are the nuclear effectors of the Hippo pathway, a central mechanism of organ size control and stem cell self-renewal. WW domains, mediators of protein-protein interactions, are essential for YAP and TAZ function, enabling interactions with PPxY motifs of numerous partner proteins. YAP has single and double WW domain isoforms (YAP1 and YAP2) whereas only a single WW domain isoform of TAZ has been described to date. Here we identify the first example of a double WW domain isoform of TAZ. Using NMR, we have characterized conformational features and peptide binding of YAP and TAZ tandem WW domains (WW1-WW2). The solution structure of YAP WW2 confirms that it has a canonical three-stranded antiparallel β-sheet WW domain fold. While chemical shift-based analysis indicates that the WW domains in the tandem WW pairs retain the characteristic WW domain fold, 15N relaxation data show that, within the respective WW pairs, YAP WW1 and both WW1 and WW2 of TAZ undergo conformational exchange. 15N relaxation data also indicate that the linker between the WW domains is flexible in both YAP and TAZ. Within both YAP and TAZ tandem WW pairs, WW1 and WW2 bind single PPxY-containing peptide ligand concurrently and noncooperatively with sub-mM affinity. YAP and TAZ WW1-WW2 bind a dual PPxY-containing peptide with approximately 6-fold higher affinity. Our results indicate that both WW domains in YAP and TAZ are functional and capable of enhanced affinity binding to multi-PPxY partner proteins such as LATS1, ErbB4, and AMOT.

The aim of the search for extraterrestrial intelligence (SETI) is to find technologically capable life beyond Earth through their technosignatures. On 2019 April 29, the Breakthrough Listen SETI project observed Proxima Centauri with the Parkes ‘Murriyang’ radio telescope. These data contained a narrowband signal with characteristics broadly consistent with a technosignature near 982 MHz (‘blc1’). Here we present a procedure for the analysis of potential technosignatures, in the context of the ubiquity of human-generated radio interference, which we apply to blc1. Using this procedure, we find that blc1 is not an extraterrestrial technosignature, but rather an electronically drifting intermodulation product of local, time-varying interferers aligned with the observing cadence. We find dozens of instances of radio interference with similar morphologies to blc1 at frequencies harmonically related to common clock oscillators. These complex intermodulation products highlight the necessity for detailed follow-up of any signal of interest using a procedure such as the one outlined in this work.

The detection of life beyond Earth is an ongoing scientific pursuit, with profound implications. One approach, known as the search for extraterrestrial intelligence (SETI), seeks to find engineered signals (‘technosignatures’) that indicate the existence of technologically capable life beyond Earth. Here, we report on the detection of a narrowband signal of interest at ~982 MHz, recorded during observations towards Proxima Centauri with the Parkes Murriyang radio telescope. This signal, BLC1, has characteristics broadly consistent with hypothesized technosignatures and is one of the most compelling candidates to date. Analysis of BLC1—which we ultimately attribute to being an unusual but locally generated form of interference—is provided in a companion paper. Nevertheless, our observations of Proxima Centauri are a particularly sensitive search for radio technosignatures towards a stellar target.

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