Between the 13th and 19th day of pregnancy the sheep conceptus developed into a structure showing considerable differentiation and all the extraembryonic membranes were established. Both length and dried weight of the embryo increased exponentially during this period. A highly significant regression of dried weight on length of embryos was found but measurement of the additional variable, width, did not improve the accuracy of estimating weight from the embryo's dimensions. The mass of the extraembryonic membranes also increased greatly. The dried weight of the trophoblast increased 90-fold over this period; that of the yolk sac increased 17-fold from day 15 to day 19. The protein content of each of the structures making up the sheep conceptus approached 50% of dried weight, which is similar to the proportion in adult soft tissues. The contribution of glycogen to dried weight was low in the sheep embryo and embryonic membranes when compared with estimates in the mouse blastocyst. However, at about the time of implantation the level of this polymer in the embryo was high compared with that in adult soft tissues and approached the level found in adult muscle. Concentrations of DNA and RNA in the sheep conceptus are much higher than the levels in most adult soft tissues and probably reflect higher synthetic rates and a smaller cytoplasmic volume in the embryonic cells.
Incorporation of glucose into the internal biochemical pools of the sheep embryo and samples of extraembryonic membranes was measured during a 2.5 h incubation in the presence of radiolabelled glucose. Very little glucose was incorporated into the glycogen pools by either the embryo or its membranes and never represented more than 5% of total incorporation. Approximately 65% of label was isolated in the non-glycogen acid-soluble fraction of samples and the remainder was incorporated into non-glycogen macromolecules. The embryonic disc of the day-13 conceptus had the highest rate of incorporation per mg dried weight of any structure studied. Synthesis of non-glycogen macromolecules by the day-13 disc was five to six times that of either day-15 or day-17 embryos. On day 19 very low rates of incorporation into the isolated embryo were found during culture. Evidence suggests that this was a result of limitations on the diffusion of substrate into the embryo because incubation of fragmented embryos produced rates similar to those found on days 15 and 17. Incorporation of glucose into the intracellular pools of extraembryonic membranes per mg dried weight remained relatively low and stable over the period studied and there were only minor differences in the rate of incorporation between membranes. Incorporation of glucose by embryos and extraembryonic membranes was equally as good in phosphate-buffered media as in bicarbonate-buffered solutions and was unaffected by changes in the concentration of lactate and pyruvate in the culture medium.
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