SummaryTops and roots of Medicago 8ativa plants raised in culture solution to which 75Se·labelled selenite or selenate had been added were shown to release 0·5-3·0% of their total selenium in volatile form when dried at 70°C for 48 hr. The volatile selenium compounds were collected on activated charcoal.Extraction of the charcoal with various solvents showed that a large part of the total volatile selenium· containing material was soluble in water but insoluble in diethyl ether. The residual moiety was soluble in ether but insoluble or sparingly soluble in water.The water·soluble fraction was shown to contain two selenium compounds which could be separated by anion-exchange column chromatography. The same two water-soluble volatile compounds were found whether selenium was supplied to the plants as selenite or selenate and whether collected from oven-drying tops or roots. The same two compounds were also obtained from intact plants.The chromatographic method used to separate these compounds also separated inorganic selenite and selenate from them and from each other.
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