Background: Hyssopus officinalis L. (hyssop), a species native to the Caspian Sea region, has been cultivated in the Republic of Moldova as aromatic plant and has been used in folk medicine as antitussive, expectorant, carminative, digestive and sedative remedy. Material and methods: The aerial parts of H. officinalis L., with pink, white and blue flowers, were collected from the collection of the Scientific Practical Center in the Field of Medicinal Plants during flowering phase. The extracts were obtained with 70% ethanol by maceration with stirring. The concetration of the extracts was done with the rotative evaporator Laborota 4011. Identification of phenolic compounds in dried extracts from hyssop herb was carried out by thin-layer chromatography. The total content of hydrocyanamic acids was measured in plant products and dried ethanolic extracts, by spectrophotometric method, with Arnow’s reagent. Quantitative analysis of total phenolic content was carried out by UV-spectrophotometry analysis, using a Metertech UV/VIS SP 8001 spectrophotometer. Results: The chlorogenic (Rf 0.47) and caffeic (Rf 0.93) acids were identified in the three genotypes of Hyssop herba, with pink, white and blue flowers. The total of hydroxycinnamic acids, expressed in caffeic acid, for both, aerial parts and dry extract, was the highest in H. officinalis L. with white flowers (1.484 mg/g; 3.014 mg/g respectively), followed by H. officinalis L. with pink flowers (1.190 mg/g; 2.915 mg/g) and H. officinalis L. with blue flowers (1.015 mg/g; 2.851 mg/g). The highest polyphenol content, expressed in gallic acid equivalent (GAE), was found in the dry extract of H. officinalis L. with blue flowers (39.056 mgGAE/g dry extract). Conclusions: This study showed that the extract of the Hyssopus officinalis L. containing phenolic compounds, can be used as a natural antioxidant in pharmaceutical and cosmetic industries.
Background: Urinary tract infections are the most common urogenital diseases, with an increased incidence in men and older people. Urogenital infections are caused by Gram-negative bacteria, in which Escherichia coli predominates with a share of 80%. The evolution of microbial resistance to preparations used in curative-prophylactic institutions, induces the need of the reintroduction of nitrofurans, noteworthy for their wide spectrum of antibacterial activity. Material and methods: For the study, suppositories with furazidine were prepared by hand rolling and by melting and molding methods. Quantitative analysis was performed spectrophotometrically on a UV-VIS Perkin Elmer Lambda 40 spectrophotometer. All solvents and reagents had the degree of purity ”pure for analysis” and ”chemically pure”. Results: Duble cast method was applied to identify the exact mass of hydrophobic (cocoa butter, suppocire) and hydrophylic (polyethylene glycol mixtures) excipients. All the formulated suppositories were subjected to quality tests and showed acceptable physical characteristics and uniformity of drug contents. The UV-VIS spectrophotometric method for quantitiative determination of furazidine was developed and validated. The validation results showed that the developed method is simple, fast, accurate and robust. Conclusions: Suppositories with furazidine were prepared by classic technological methods. Preparation of the suppositories with furazidine on cocoa butter excipient is a suitable alternative for individual medicinal prescriptions. The UV-VIS spectrophotometric dosing method for furazidine in suppositories was developed and validated
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] M 43Abstracts. PHYTOPHARM 2012Artichoke C. scolymus l. is a medicinal plant frequently used in folk and scientific medicine due to its therapeutic properties, including antioxidant effects, hepatoprotective ability and cholesterol-lowering effects. The aim of the work was to evaluate the morphoanatomical adaptations of the artichoke to the environmental conditions of the Republic of Moldova that differ from countries of plant origin. The morpho-anatomical investigations were effectuated on the artichokes grown in the Collection of Medicinal Plants of the Centre for the Cultivation of Medicinal plants of our University. Were carried out morpho-biometrical evaluation of the artichoke plants and anatomical study of their leaves. The morpho-biometrical evaluation of the artichoke was effectuated on more that 10 parameters: plant height, steam diameter, number of branches, foliar levels, number of leaves on a plant, leaf length, leaf width, number of leaf lobes, flower heads on a plant, flower heads on a branch, flower heads diameter, also leaf area was estimated. The statistic evaluation shown that the principle biometrical parameters are average plant height -85,5 ± 5,2 cm foliar levels -12,0 ± 0,8 number of leaves on a plant -20,0 ± 1,1 average leaf area -146,7 cm . Anatomical study of artichoke leaf elucidated specific anatomical structures: glandular and 2 types of covering trichomes on both epiderma, dorsoventral structure of mesophyll, anomocytic stomata, collateral vascular bundles and angular collenchyma. in conclusion the morpho-biometric results in correlation with anatomical data demonstrate that C.scolymus l. grow and develop well in the climatic conditions of Republic of Moldova and can be targeted for further phytochemical analysis. DETERMINATION OF THE TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENTS IN CYNARA SCOLYMUS L. EXTRACTS © Ciobanu Cristina State Medical and Pharmaceutical University "Nicolae Testemiţanu", Faculty of Pharmacy, Chisinau, Republic of MoldovaArtichoke -Cynara scolymus l. is a plant widely cultivated in Europe and the United States effective on the treatments of hepatobiliary diseases, hyperlipidaemia and cholesterol metabolism (1). The purpose of the study was to determine the main biological active compounds in leaf and flower heads extracts obtained from artichokes grown in Centre for the Cultivation of Medicinal plants of The State Medical and Pharmaceutical University "Nicolae Testemiţanu". determination of total phenol and total flavonoid contents in C. solymus l. leaves and flower heads extracts in different concentrations of the ethanol solvent used was carried out using spectrophotometric methods. The total phenolic content was determinated by using cynarin (1,3-dicaffeoylquinic acid) as reference material, in the 360 nm wavelength, calibration curve for the analyte was linear with the correlation coefficients (y = 51,572x, R2 = 0.9973). Total flavonoid determination...
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] M 43Abstracts. PHYTOPHARM 2012Artichoke C. scolymus l. is a medicinal plant frequently used in folk and scientific medicine due to its therapeutic properties, including antioxidant effects, hepatoprotective ability and cholesterol-lowering effects. The aim of the work was to evaluate the morphoanatomical adaptations of the artichoke to the environmental conditions of the Republic of Moldova that differ from countries of plant origin. The morpho-anatomical investigations were effectuated on the artichokes grown in the Collection of Medicinal Plants of the Centre for the Cultivation of Medicinal plants of our University. Were carried out morpho-biometrical evaluation of the artichoke plants and anatomical study of their leaves. The morpho-biometrical evaluation of the artichoke was effectuated on more that 10 parameters: plant height, steam diameter, number of branches, foliar levels, number of leaves on a plant, leaf length, leaf width, number of leaf lobes, flower heads on a plant, flower heads on a branch, flower heads diameter, also leaf area was estimated. The statistic evaluation shown that the principle biometrical parameters are average plant height -85,5 ± 5,2 cm foliar levels -12,0 ± 0,8 number of leaves on a plant -20,0 ± 1,1 average leaf area -146,7 cm . Anatomical study of artichoke leaf elucidated specific anatomical structures: glandular and 2 types of covering trichomes on both epiderma, dorsoventral structure of mesophyll, anomocytic stomata, collateral vascular bundles and angular collenchyma. in conclusion the morpho-biometric results in correlation with anatomical data demonstrate that C.scolymus l. grow and develop well in the climatic conditions of Republic of Moldova and can be targeted for further phytochemical analysis. DETERMINATION OF THE TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENTS IN CYNARA SCOLYMUS L. EXTRACTS © Ciobanu Cristina State Medical and Pharmaceutical University "Nicolae Testemiţanu", Faculty of Pharmacy, Chisinau, Republic of MoldovaArtichoke -Cynara scolymus l. is a plant widely cultivated in Europe and the United States effective on the treatments of hepatobiliary diseases, hyperlipidaemia and cholesterol metabolism (1). The purpose of the study was to determine the main biological active compounds in leaf and flower heads extracts obtained from artichokes grown in Centre for the Cultivation of Medicinal plants of The State Medical and Pharmaceutical University "Nicolae Testemiţanu". determination of total phenol and total flavonoid contents in C. solymus l. leaves and flower heads extracts in different concentrations of the ethanol solvent used was carried out using spectrophotometric methods. The total phenolic content was determinated by using cynarin (1,3-dicaffeoylquinic acid) as reference material, in the 360 nm wavelength, calibration curve for the analyte was linear with the correlation coefficients (y = 51,572x, R2 = 0.9973). Total flavonoid determination...
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