This study aimed to investigate the effects of different feeding patterns on meat quality, gut microbiota and its metabolites of Tibetan pigs. Tibetan pigs with similar body weight were fed the high energy diets (HEP, 20 pigs) and the regular diets (RFP, 20 pigs), and free-ranging Tibetan pigs (FRP, 20 pigs) were selected as the reference. After 6 weeks of experiment, meat quality indexes of semitendinosus muscle (SM) and cecal microbiota were measured. The results of meat quality demonstrated that the shear force of pig SM in FRP group was higher than that in HEP and RFP groups (p < 0.001); the pH-value of SM in HEP pigs was higher at 45 min (p < 0.05) and lower at 24 h (p < 0.01) after slaughter than that in FRP and RFP groups; the SM lightness (L* value) of FRP pigs increased compared with RFP and HEP groups (p < 0.001), while the SM redness (a* value) of FRP pigs was higher than that of RFP group (p < 0.05). The free fatty acid (FA) profile exhibited that the total FAs and unsaturated FAs of pig SM in HEP and RFP groups were higher than those in FRP group (p < 0.05); the RFP pigs had more reasonable FA composition with higher n-3 polyunsaturated FAs (PUFAs) and lower n-6/n-3 PUFA ratio than HEP pigs (p < 0.05). Based on that, we observed that Tibetan pigs fed high energy diets (HEP) had lower microbial α-diversity in cecum (p < 0.05), and distinct feeding patterns exhibited a different microbial cluster. Simultaneously, the short-chain FA levels in cecum of FRP and RFP pigs were higher compared with HEP pigs (p < 0.05). A total of 11 genera related to muscle lipid metabolism or meat quality, including Alistipes, Anaerovibrio, Acetitomaculun, etc., were identified under different feeding patterns (p < 0.05). Spearman correlation analysis demonstrated that alterations of free FAs in SM were affected by the genera Prevotellaceae_NK3B31_group, Prevotellaceae UCG-003 and Christensenellaceae_R-7_group (p < 0.05). Taken together, distinct feeding patterns affected meat quality of Tibetan pigs related to gut microbiota alterations.
Yaks usually live in an extremely harsh natural environment resulting in low reproductive performance, so the production of yak cannot meet local demand in China. In order to solve this problem, the experiment aims to explore the effect of different feeding modes on the semen quality of male yaks, so as to provide a theoretical basis for improving the yield of yaks in Tibet. We used the combined analysis of metabolomics and microbial sequencing to explore the underlying mechanisms that affect the differences in semen quality between the house feeding (HF) system and the free range (FR). The results showed that the sperm motility (P < 0.001) and sperm concentration (P < 0.05) in the HF group were significantly higher than the FR group, and the abnormal sperm rate (P < 0.01) in HF was significantly lower compared to FR. House feeding modes increased some beneficial materials in blood and testis especially some antioxidants, unsaturated fatty acids, and amino acids. House feeding group increased some gut microbiota at genus level namely Rikenellaceae, Bacteroides, Prevotellaceae_UCG-004, Bacteroidales_RF16, and Alloprevotella, DgA-11. It was interesting that blood metabolites, testicular metabolites, and fecal microbiota were well-correlated with sperm parameters. Meanwhile, the blood metabolites and testicular metabolites were well-correlated with microbes. The result indicated that the HF model was beneficial for yak semen quality by improving the gut microbiota and blood metabolism to increase yak fertility.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.