Particulate nitrate (pNO3 –) has often been found to be the major component of fine particles in urban air-sheds in China, the United States, and Europe during winter haze episodes in recent years. However, there is a lack of knowledge regarding the experimentally determined contribution of different chemical pathways to the formation of pNO3 –. Here, for the first time, we combine ground and tall-tower observations to quantify the chemical formation of pNO3 – using observationally constrained model approach based on direct observations of OH and N2O5 for the urban air-shed. We find that the gas-phase oxidation pathway (OH+NO2) during the daytime is the dominant channel over the nocturnal uptake of N2O5 during pollution episodes, with percentages of 74% in urban areas and 76% in suburban areas. This is quite different from previous studies in some regions of the US, in which the uptake of N2O5 was concluded to account for a larger contribution in winter. These results indicate that the driving factor of nitrate pollution in Beijing and different regions of the US is different, as are the mitigation strategies for particulate nitrate.

Atherosclerosis is a main reason for peripheral vascular disease. The present study aims to investigate the effects of macrophage foam cells which is an initial part in atherosclerosis. RAW 264.7 were treated with 80 μg/mL oxidized low-density lipoproteins (ox-LDL) to mimic atherosclerosis in vitro. Orientin, a flavonoid from plants, inhibited ox-LDL induced TNFα, IL-6, IL-1β expression increase. In addition, Orientin also can inhibit the emergence of ox-LDL-induced lipid droplets. The scavenger receptor CD 36 of ox-LDL was significantly downregulated after the treatment of orientin. Inhibition of ROS generation and increasing of eNOS expression by Orientin treatment was used to show the alteration of oxidative stress. Moreover, the expression levels of Angiopoietin-like 2 (angptl2) and NF-κB were significantly upregulated after cells induced by ox-LDL, whereas orientin significantly reversed the effects of ox-LDL. Orientin inhibited ox-LDL-induced inflammation and oxidative stress, and CD36 may be the key regulator during Orientin action.

Pyruvate kinase (PK) M2 (PKM2), a glycolytic enzyme, is a hallmark of different types of tumors and plays a significant role in the Warburg effect. However, there is no fluorescent probe for PKM2 that has been reported yet. In this study, TEPC466, a novel TEPP-46-based aggregation-induced emission (AIE) probe for the detection of PKM2, was designed, synthesized, and fully characterized by 1H NMR, 13C NMR, and high-resolution mass spectrometry. When the fluorescent agent, coumarine, was conjugated to TEPP-46, the bioprobe TEPC466 showed a high degree of selectivity and sensitivity for the detection of PKM2 protein via the AIE effect. TEPC466 was then successfully applied in imaging the PKM2 protein in colorectal cancer cells with low toxicity. Moreover, structure-based modeling and the PK activity assay confirmed that TEPC466 has a better binding with PKM2 than TEPP-46, which suggests that TEPC466 could also be a good agonist of PKM2. Taken together, the bioprobe shows potential in selective detection of PKM2 and provides a useful tool for cancer diagnosis and therapy.

BackgroundThis study aimed to evaluate the early and mid-term outcomes of drug-coated balloon (DCB) use in patients who underwent intervention for transplant renal artery stenosis (TRAS).Material/MethodsWe retrospectively reviewed the records of TRAS patients who received endovascular therapy with DCB in our institution from March 2016 to January 2017. Statistical analysis of pre-/postoperative levels of serum creatinine (Scr), systolic blood pressure (SBP), and renal artery peak systolic velocities (PSV) were performed.ResultsFourteen patients presenting with TRAS, which were mostly located at the anastomosis (n=9) and transplanted artery proximal portion (n=2), were treated with DCB. Three TRAS patients with in-stent restenosis (ISR) were also included in the series. The procedure technique success rate was 100%. The mean follow-up time was 8.6 months. The Scr level decreased from 481.8 μmol/L (208.5–746.2μmol/L) pre-operation to 154μmol/L (89.1–301.2 μmol/L, p<0.01) at 1 month post-intervention. The SBP varied from 161.4 mmHg (152–173 mmHg) to 144.8 mmHg (136–154 mmHg, p<0.01). Renal artery PSV decreased from 364.1 cm/s (217.6–511.9 cm/s) to 134.9 cm/s (79.8–184.2 cm/s, p<0.01). Eleven patients finished mid-term (>6 months) follow-up. The statistical results were not significant compared to those at 1 month, although they all slightly decreased. No re-intervention was performed.ConclusionsThe endovascular approach to TRAS with DCB was a safe and effective treatment for restore and maintain the artery flow and renal function in short-term follow-up.