Pulsed electric fields (PEF) as a new pasteurization technology played an important role in the process of inactivating microorganisms. At the same time, temperature could promote the process of electroporation, and achieve better inactivation effect. This article studied the inactivation effect of PEF on Saccharomyces cerevisiae, Escherichia coli, and Bacillus velezensis under different initial temperatures (room temperature-24 $$\mathrm{^\circ{\rm C} }$$
∘
C
, 30 $$\mathrm{^\circ{\rm C} }$$
∘
C
, 40 $$\mathrm{^\circ{\rm C} }$$
∘
C
, 50 $$\mathrm{^\circ{\rm C} }$$
∘
C
). From the inactivation results, it found temperature could reduce the critical electric field intensity for microbial inactivation. After the irreversible electroporation of microorganisms occurred, the nucleic acid content and protein content in the suspension increased with the inactivation rate because the cell membrane integrity was destroyed. We had proved that the electric field and temperature could promote molecular transport through the finite element simulation. Under the same initial temperature and electrical parameters (electric field intensity, pulse width, pulse number), the lethal effect on different microorganisms was Saccharomyces cerevisiae > Escherichia coli > Bacillus velezensis.
A dielectric barrier discharge (DBD) reactor is introduced to generate H 2 O 2 by non-thermal plasma with a mixture of oxygen and water mist produced by an ultrasonic atomizer. The results of our experiment show that the energy yield and concentration of the generated H 2 O 2 in the pulsed discharge are much higher than that in AC discharge, due to its high energy efficiency and low heating effect. Micron-sized liquid droplets produced by an ultrasonic atomizer in water mist have large specific surface area, which greatly reduces mass transfer resistance between hydroxyl radicals and water liquids, leading to higher energy yield and H 2 O 2 concentration than in our previous research. The influence of applied voltage, discharge frequency, and environmental temperature on the generated H 2 O 2 is discussed in detail from the viewpoint of the DBD mechanism. The H 2 O 2 concentration of 30 mg l −1 , with the energy yield of 2 g kW −1 h −1 is obtained by pulsed discharge in our research.
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