In the traditional fermentation process of strong-aroma Baijiu, a fermentation pit mud (FPM) provides many genera of microorganisms for fermentation. However, the functional microorganisms that have an important effect on the quality of Baijiu and their changes with the age of fermentation pit (FP) are poorly understood. Herein, the Roche 454 pyrosequencing technique and a phospholipid fatty-acid analysis were employed to reveal the structure and diversity of prokaryotic communities in FPM samples that have been aged for 5, 30, and 100 years. The results revealed an increase in total prokaryotic biomass with an FP age; however, Shannon’s diversity index decreased significantly (p < 0.01). These results suggested that a unique microbial community structure evolved with uninterrupted use of the FP. The number of functional microorganisms, which could produce the flavor compounds of strong-aroma Baijiu, increased with the FP age. Among them, Clostridium and Ruminococcaceae are microorganisms that directly produce caproic acid. The increase of their relative abundance in the FPM might have improved the quality of strong-aroma Baijiu. Syntrophomonas, Methanobacterium, and Methanocorpusculum might also be beneficial to caproic acid production. They are not directly involved but provide possible environmental factors for caproic acid production. Overall, our study results indicated that an uninterrupted use of the FP shapes the particular microbial community structure in the FPM. This research provides scientific support for the concept that the aged FP yields a high-quality Baijiu.
In this account, the interaction between E44 and KH560 modifiers and poly(butylene succinate)‐distillers grains (PBS/DGs) composite system was studied experimentally and theoretically by means of molecular dynamics simulation. The PBS/DG biodegradable composites were prepared by melt blending the modified DG and PBS, and composting degradation tests were carried out for a period of 75 days. The effect of E44 and KH560 modifiers on the interfacial features, tensile characteristics, thermal stability and degradation properties of PBS/DG composites were examined. The results showed that both χ and Emix significantly decreased in the composite system, indicating that PBS and DG became easier to mix together in presence of E44 and KH560. Also, the interface effect of DG and PBS improved after the modification with KH560. The mechanical properties of the composites remarkably enhanced when DG was treated with 2% KH560. When compared with PBS, the tensile strength and elongation at break were boosted by 23.66 and 33.18%, respectively. After composting degradation for 75 days, the mass loss rate of PBS/DG reached 42.82%, corresponding to 5.90 times higher than that of pure PBS. Overall, the comprehensive properties of the composites improved. POLYM. COMPOS., 40:1499–1509, 2019. © 2018 Society of Plastics Engineers
Endometriosis (EM) with chronic inflammation may accelerate the progression of atherosclerosis. Currently, no large or randomized clinical studies have assessed the incidence of cardiovascular events in patients with endometriosis in Asia to investigate whether incident EM is associated with a higher risk of new-onset coronary artery disease (CAD). In this study of a nationwide cohort in Taiwan, we identified 13,988 patients with newly diagnosed EM from 1 January, 2000, through 31 December, 2012. EM and non-EM groups were matched by propensity score at a ratio of 1:1. Of a total 27,976 participants, 358 developed CAD. The incidence rate in the EM group was higher than that in the non-EM group (1.8 per 1,000 person-years vs. 1.3 per 1,000 person-years) during the follow-up period. The adjusted hazard ratio (aHR) of CAD for the EM group was 1.52 with a 95% confidence interval (1.23–1.87, p < 0.001) after adjusting for demographic characteristics, comorbidities, surgical procedures, frequency of outpatient visits, and medications. Stratified analysis revealed that, among four age groups (20–39, 40–49, 50–54, and above 55 years), the 20–39 years sub-group was associated with a higher risk of CAD (aHR, 1.73; 95% CI, 1.16–2.59, p = 0.008). Several sensitivity analyses were conducted for cross-validation, and it showed consistent positive findings. In conclusion, this cohort study revealed that patients with symptomatic EM in Taiwan were associated with increased risk of subsequent CAD than patients without medical records of EM. Further prospective studies are needed to confirm this causal relationship.
Adsorbent material was prepared using distillers’ grains (DG), which is a waste product of distilleries. The DG was pre-treated with NaOH and esterification-modified with CS2, which is a commonly used anionic modifier. The structure and morphology of the adsorbent was characterized by FTIR, XRD, EDS, SEM, BET, and zeta potential. The related mechanism of adsorption of malachite green (MG) onto modified distiller’s grains (MDG) was studied by adsorption experiments and molecular simulation techniques. The experimental results showed that CS2 successfully modified the DG fiber, and simultaneously yielded the MDG with a uniform pore distribution. MDG had a considerable adsorption capacity of 367.39 mg/g and a maximum removal rate of 96.51%. After eight adsorption–desorption cycle experiments, the adsorption removal rate of MDG to MG dye remained at 82.6%. The adsorption process could be fitted well by a pseudo-second-order kinetic model (the correlation coefficient R2 > 0.998) and Freundlich isotherm adsorption equation (the correlation coefficient R2 > 0.972). Moreover, the adsorption of MG dye by MDG is a spontaneous, endothermic, and increased entropy process. The results of molecular simulation showed that the mechanism of MG molecules onto MDG was mainly chemical adsorption. The adsorption performance of MG onto MDG was better and more stable than DG. Molecular simulation also provided a theoretical guidance of MDG adsorption–desorption for the research on recycling of DG resources.
Using distillers grains (DG) as raw material after pre-treatment with sodium hydroxide (NaOH) and modified with cationic etherification agent 3-chloro-2-hydroxypropyltrimethylammonium chloride (CHPTAC), cationic quaternary ammonium distillers grains adsorption material (CDG) was successfully prepared. The optimal adsorption conditions were an adsorption temperature of 25 °C, adsorption time of 180 min, amount of adsorbent at 8.5 g/L, initial dye concentration of 100 mg/L, and pH of dye solution 7.0. The structure of CDG was characterized by FTIR, EDS, SEM, BET, ultraviolet spectrum analysis, and analysis of the zeta potential, while the adsorption mechanism was studied by adsorption kinetics, isotherms, and thermodynamics. The results showed that CHPTAC modified the distillers grains successfully and induced the formation of CDG with a large number of pore structures and good adsorption effect. The highest adsorption yield was above 98%, while after eight rounds of adsorption–desorption experiments, the adsorption rate was 81.80%. The adsorption mechanism showed that the adsorption process of acid yellow 11 (AY11) by CDG conforms to the pseudo-second-order kinetic model, mainly with chemical and physical adsorption such as pore adsorption and electrostatic adsorption. Thermodynamics conforms to the Freundlich isothermal model, and the adsorption process is a spontaneous, endothermic and entropy-increasing process.
Distilled jujube liquor is an alcoholic beverage made from jujube, which has a unique flavor and a sweet taste. The purpose of this study was to explore the effect of mixed fermentation on the quality of distilled jujube liquor by comparing the performance of mixed fermentation between S. cerevisiae, Pichia pastoris and Lactobacillus. The results showed that there were significant differences in the quality of the jujube liquor between the combined strains. Moreover, Lactobacillus increased and P. pastoris reduced the total acid content. The results from an E-nose showed that the contents of methyl, alcohol, aldehyde, and ketone substances in the test bottle decreased significantly after decanting, while the contents of inorganic sulfide and organic sulfide increased. Fifty flavor compounds were detected, including nineteen esters, twelve alcohols, seven ketones, six aldehydes, three alkenes, one furan, one pyridine, and one acid. There were no significant differences in the type or content of flavor compounds. However, PLS-DA showed differences among the samples. Eighteen volatile organic compounds with variable importance in projection values > 1 were obtained. There were sensory differences among the four samples. Compared with the sample fermented with only S. cerevisiae, the samples co-fermented with Lactobacillus or with P. pastoris had an obvious bitter taste and mellow taste, respectively. The sample fermented by all three strains had a prominent fruity flavor. Except for the sample fermented with only S. cerevisiae, the jujube flavor was weakened to varying degrees in all samples. Co-fermentation could be a valuable method to improve the flavor quality of distilled jujube liquor. This study revealed the effects of different mixed fermentation modes on the sensory flavor of distilled jujube liquor and provided a theoretical basis for the establishment of special mixed fermentation agents for distilled jujube liquor in the future.
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