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Helicobacter pylori infection is linked to serious gastric-related diseases including gastric cancer. However, current therapies for treating H. pylori infection are challenged by the increased antibiotic resistance of H. pylori. Therefore, it is in an urgent need to identify novel targets for drug development against H. pylori infection. In this study, HP0860 gene from H. pylori predicted to encode a D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) involved in the synthesis of ADP-L-glycero-D-manno-heptose for the assembly of lipopolysaccharide (LPS) in the inner core region was cloned and characterized. We reported HP0860 protein is monomeric and functions as a phosphatase by converting D-glycero-D-manno-heptose-1,7-bisphosphate into D-glycero-D-manno-heptose-1-phosphate with a preference for the β-anomer over the α-anomer of sugar phosphate substrates. Subsequently, a HP0860 knockout mutant and its complementary mutant were constructed and their phenotypic properties were examined. HP0860 knockout mutant contained both mature and immature forms of LPS and could still induce significant IL-8 secretion after gastric AGS cell infection, suggesting other enzymatic activities in HP0860 knockout mutant might be able to partially compensate for the loss of HP0860 activity. In addition, HP0860 knockout mutant was much more sensitive to antibiotic novobiocin, had decreased adherence abilities, and caused less classic hummingbird phenotype on the infected AGS cells, indicating H. pylori lacking HP0860 is less virulent. Furthermore, the disruption of HP0860 gene altered the sorting of cargo proteins into outer membrane vesicles (OMVs). The above findings confirm the importance of HP0860 in LPS core biosynthesis and shed light on therapeutic intervention against H. pylori infection.

A fully experimental modeling technique and a design optimization procedure are presented in this paper for push-pull electret loudspeakers. Conventional electrical impedance-based parameter identification methods are not completely applicable to electret speakers due to the extremely weak electromechanical coupling. This prompts the development of an experimental technique for identifying the electroacoustic parameters of the electret speakers. Mechanical parameters are identified from the membrane velocity measured using a laser vibrometer. The voltage-force conversion factor and the motional impedance are estimated, with the aid of a test-box method. This experimentally identified model serves as the simulation platform for predicting the response of the electret loudspeaker and optimizing the design. Optimal parameters are calculated by using the simulated annealing (SA) algorithm to fulfill various design goals and constraints. Either the comprehensive search for various parameters or the simple search for the optimal gap distance can be conducted by this SA procedure. The results reveal that the optimized design has effectively enhanced the performance of the electret loudspeaker.

An unconventional type of electrostatic loudspeaker is presented in this paper. The loudspeaker made of thin, light, and flexible electret material lends itself well to the space-concerned applications. Electrical impedance measurement reveals that the coupling between the electrical system and the mechanical system is weak, which renders conventional parameter identification based on electrical impedance measurement impractical. A different approach is thus employed to model the electret loudspeaker. To predict the loudspeaker's dynamic response, finite-element analysis (FEA) is conducted on the basis of a simple model and a full model. In the simple model, FEA is applied to model the electret membrane, leaving the rest of system as rigid parts. In the full model, FEA is applied to model the entire membrane-spacer-back plate assembly. Velocity response of the membrane subject to a uniformly distributed force is calculated using FEA harmonic analysis. Mechanical impedance is then calculated with the velocity response. The acoustical impedance due to the back cavity, pores, and the radiation loading at the front side is calculated by theoretical formulas. The volume velocity of the membrane and the resulting on-axis sound pressure level are predicted with electrical-mechanical-acoustical analogous circuits. The response data predicted by the simulation compare very well with experimental measurements.