BackgroundMajor secondary metabolites, including flavonoids, caffeine, and theanine, are important components of tea products and are closely related to the taste, flavor, and health benefits of tea. Secondary metabolite biosynthesis in Camellia sinensis is differentially regulated in different tissues during growth and development. Until now, little was known about the expression patterns of genes involved in secondary metabolic pathways or their regulatory mechanisms. This study aimed to generate expression profiles for C. sinensis tissues and to build a gene regulation model of the secondary metabolic pathways.ResultsRNA sequencing was performed on 13 different tissue samples from various organs and developmental stages of tea plants, including buds and leaves of different ages, stems, flowers, seeds, and roots. A total of 43.7 Gbp of raw sequencing data were generated, from which 347,827 unigenes were assembled and annotated. There were 46,693, 8446, 3814, 10,206, and 4948 unigenes specifically expressed in the buds and leaves, stems, flowers, seeds, and roots, respectively. In total, 1719 unigenes were identified as being involved in the secondary metabolic pathways in C. sinensis, and the expression patterns of the genes involved in flavonoid, caffeine, and theanine biosynthesis were characterized, revealing the dynamic nature of their regulation during plant growth and development. The possible transcription factor regulation network for the biosynthesis of flavonoid, caffeine, and theanine was built, encompassing 339 transcription factors from 35 families, namely bHLH, MYB, and NAC, among others. Remarkably, not only did the data reveal the possible critical check points in the flavonoid, caffeine, and theanine biosynthesis pathways, but also implicated the key transcription factors and related mechanisms in the regulation of secondary metabolite biosynthesis.ConclusionsOur study generated gene expression profiles for different tissues at different developmental stages in tea plants. The gene network responsible for the regulation of the secondary metabolic pathways was analyzed. Our work elucidated the possible cross talk in gene regulation between the secondary metabolite biosynthetic pathways in C. sinensis. The results increase our understanding of how secondary metabolic pathways are regulated during plant development and growth cycles, and help pave the way for genetic selection and engineering for germplasm improvement.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1773-0) contains supplementary material, which is available to authorized users.

Formic acid is a highly fatal source of carbon monoxide poisoning when mixed with sulfuric acid. In addition to the toxicities of carbon monoxide, concomitant inhalation of formic acid fumes can cause severe lung injury, which may complicate the management of carbon monoxide poisoning.

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The optical and electrical properties of Tin oxide (SnO2) tranparent conducting film are related to its structural properties. The films were grown by chemical vapor deposition on four different substrates at atmospheric pressure and temperatures of 420°C to investigate the effects of substrate material and surface roughness on their structure. A homemade vertical reactor was used for deposition from SnCl4 + 2H2O precursors. The structure were analyzed by X-ray diffraction (XRD), scanning electron microscopy( SEM). The results indicated that substrate material influenced the crystal size, compactedness and predominant planes of SnO2 films while surface roughness influenced crystallization and compactedness of the films.

In the context of the era of speed economic, the airport as a quick means of transportation, promote the rapid flow of local people, capital, technology and other factors of production, promote local economic development, and development of the airport is also inseparable from the support of the local economy, Therefore, Use the airport and local economic data for data processing,and analyzing the degree of coupled development of airport and local economic scientifically, planning for further improving coordination between the airport and the city, and promoting coordinated development between them is important.

High-speed train air-conditioning systems put forward higher request on the aspect of air uniformity and comfort. The use of orifice which can form a more uniform velocity and temperature field in the car can be widely applied to high-speed train air-conditioning system. The experimental study of the influence of several different factors (such as the opening rate, aperture, orifice plate thickness, etc.) in the research may provide a reference for the design choices of the orifice type.