The effect of substrate (medium) on growth, yield and nutritional composition of domestically-grown oyster mushroom (Pleurotus ostreatus) was investigated. Six different substrates namely sawdust only (SDO), sawdust + corn waste + CaCO 3 (SDW), sawdust + rice bran + CaCO 3 (SDR), sawdust + banana leaves (SBL), sawdust + cassava peel (SDC) and cassava peel only (CPO) were used. The substrates were pasteurized with hot water (90°C for 4 h) before spawns of oyster mushroom were inoculated to them. After inoculation, the substrates were kept in a controlled environment until fruiting took place. The SDC substrate gave the highest number (22) of fruiting body, highest yield (463 g/kg) and best biological efficiency (46.30%). This was followed closely by the harvest from SDR substrate. The differences in the nutrient composition of mushroom from the different substrates were significant at 0.05 % confidence level. Harvest from SDR contained higher vitamins and minerals compared to others. Harvest from CPO substrate had the lowest (20.10%) protein content as well as other nutrients. SDC and SDR substrates are considered good for domestic cultivation of oyster mushroom.
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