Hempseed is a nutrient-rich natural resource, and high levels of hempseed oil accumulate within hemp seeds, consisting primarily of different triglycerides. Members of the diacylglycerol acyltransferase (DGAT) enzyme family play critical roles in catalyzing triacylglycerol biosynthesis in plants, often governing the rate-limiting step in this process. As such, this study was designed to characterize the Cannabis sativa DGAT (CsDGAT) gene family in detail. Genomic analyses of the C. sativa revealed 10 candidate DGAT genes that were classified into four families (DGAT1, DGAT2, DGAT3, WS/DGAT) based on the features of different isoforms. Members of the CsDGAT family were found to be associated with large numbers of cis-acting promoter elements, including plant response elements, plant hormone response elements, light response elements, and stress response elements, suggesting roles for these genes in key processes such as development, environmental adaptation, and abiotic stress responses. Profiling of these genes in various tissues and varieties revealed varying spatial patterns of CsDGAT expression dynamics and differences in expression among C. sativa varieties, suggesting that the members of this gene family likely play distinct functional regulatory functions CsDGAT genes were upregulated in response to cold stress, and significant differences in the mode of regulation were observed when comparing roots and leaves, indicating that CsDGAT genes may play positive roles as regulators of cold responses in hemp while also playing distinct roles in shaping the responses of different parts of hemp seedlings to cold exposure. These data provide a robust basis for further functional studies of this gene family, supporting future efforts to screen the significance of CsDGAT candidate genes to validate their functions to improve hempseed oil composition.
The wheel tractor driving simulator was developed by using desktop virtual reality technology to solve the problems of short training times and complex operation for drivers, which led to machine damage and low efficiency when the wheel tractor was used. Firstly, the composition of the tractor driving simulator and its working principle were presented. Secondly, combined with the obtained parameters and operation requirements, the key components of the wheeled tractor driving simulator were designed. Finally, the high-degree simulation of the wheeled tractor driving simulator was verified by comparing the parameters of the key components of the real tractor with those of the wheeled tractor driving simulator. The test results showed the stroke angle and feedback force of the simulator were basically the same as that of the real tractor and they had the same trends. Therefore, the key components of the wheeled tractor driving simulator designed in this paper can simulate the driving body sense and operational functions of real tractors. The development of a driving simulator is of great significance to train unqualified drivers, improve training efficiency and reduce training cost.
Abstract. In order to improve authenticity and interactive of the tractor driving simulator system, mainly adopts double polarity 555 chip design for tractor driving simulator of turn signal circuit. Then specify bipolar 555 chip unsteady work mode setting method and principle ,and gives the tractor simulator turn signal circuit connection method.Using the method of tractor simulator turn signal circuit has simple structure, low price, etc. After the computer simulation, the result shows that output waveform of square wave signals, flicker frequency is 84 times/min, meet the design requirements.
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