Previously, we demonstrated that a submerged fermentation culture of Antrodia camphorata (AC) promotes cell-cycle arrest and apoptosis in human estrogen receptor-positive/negative breast cancer cells. However, whether AC is effective against HER-2/neu-overexpressing breast cancers has not been thoroughly elucidated. In the present study, we showed that AC exhibited a significant cytotoxic effect against HER-2/neu-overexpressing MDA-MB-453 and BT-474 cells. Immunoblot analysis demonstrated that HER-2/neu and their tyrosine phosphorylation were inhibited by AC in a dose-dependent manner. An increase in intracellular reactive oxygen species (ROS) was observed in AC-treated cells, whereas antioxidant N-acetylcysteine (NAC) significantly prevented AC induced HER-2/neu depletion and cell death, which directly indicates that AC-induced HER-2/neu depletion and cell death was mediated by ROS generation. Also, AC significantly downregulated the expression of cyclin D1, cyclin E, and CDK4 followed by the suppression of PI3K/Akt, and their downstream effectors GSK-3β and β-catenin. Notably, AC-treatment induced apoptotic cell death, which was associated with sub-G1 accumulation, DNA fragmentation, mitochondrial dysfunction, cytochrome c release, caspase-3/-9 activation, PARP degradation, and Bcl-2/Bax dysregulation. Assays for colony formation also confirmed the growth-inhibitory effects of AC. This is the first report confirming the anticancer activity of this potentially beneficial mushroom against human HER-2/neu-overexpressing breast cancers.
Descriptive statistics and Scheffe multiple comparison analysis by all planting the average temperature difference at the 0.05 level values are significant. The temperature difference between the control as the dependent variable, more independent variables, and found that different types of solar radiation energy planting isolated cooling there are significant , the bestRhoeo spathaceadown 13.7226°C,Orthosiphon aristatusdown12.0540°C,followed byCynodonspp. down 8.0591°C least significant cooling.
In this study, the use waste of natural fiber applied in building facades and green roofs vegetation materials to reduce production costs and price, prompting facade green roof vegetation universal use, reduce the heat island effect to improve indoor and outdoor microclimate environment and ecological house in Taiwan, ecological communities, ecological city to create an inject vitality. The media of planting Cynodon spp. and Eremochloa ophiuroides material through quantitative data analysis and comparison of the 3 groups selected on material that suits the media light (670g), saturated moisture content (218%), low-cost ($1.1 USD). Planting Angelonia salicariifolia,Orthosiphon aristatus,Barleria repens material 17 group, through quantitative data analysis and comparison of the 3 groups selected of material that suits the media light (950g), saturated moisture content (241%), low-cost ($1.4 USD).
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