Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1Mammalian centrosomes separate prior to mitosis in a process involving Cdk1 and the kinesin Eg5. Dissection by chemical genetics reveals a Cdk1-independent alternative mechanism, in which Plk1 counteracts both centrosomal cohesion factors and Eg5-opposing cytoskeletal forces.

Background: Tau aggregation inhibitors could treat Alzheimer disease.Results: Stable reduced forms of leucomethylthioninium (LMTX®) are active in blocking prion-like Tau aggregation in novel cellular models.Conclusion: The intracellular Ki (0.12 μm) is comparable with brain levels required for clinical benefit.Significance: LMTX® could treat Alzheimer disease.

The reaction of UI3 and KCpRR' (CpRR' = pentamethylcyclopentadienyl, trimethylsilylcyclopentadienyl or tetramethylcyclopentadienyl) in diethyl ether results in the two-electron reduction of the solvent to form trimetallic, mixed valence uranium oxo species.

Formaldehyde is commonly used as an alkylating agent in the pharmaceutical industry. Consequently, its residual level in drug substances and/or their intermediates needs to be accurately quantified. Formaldehyde is a small, volatile molecule with a weak chromophore (the carbonyl group), and its direct analysis by GC-FID and HPLC-UV is difficult. For these reasons, the majority of papers found in the literature are based upon a derivatisation process (most commonly using the desensitised explosive 2,4-dinitrophenylhydrazine) prior to the analysis of formaldehyde. A novel high-performance liquid chromatography (HPLC) method with UV detection for its quantification in a pharmaceutical is described in this paper. The method proposed herein is based upon a derivatisation reaction between formaldehyde and 4-methylbenzenesulfonohydrazide (MBSH) before analysis by HPLC-UV. Selectivity, linearity, limit of quantification, accuracy, repeatability, intermediate precision, and solution stability were successfully assessed as per ICH guideline Q2(R1), and the method has also been validated in a good manufacturing practice (GMP) laboratory in the UK.