Objectives: Little research exists documenting levels of intestinal inflammationamong indigenous populations where exposure to macroparasites, like soil-transmitted helminths (STHs), is common. Reduced STH exposure is hypothesized to contribute to increased prevalence of elevated intestinal inflammation in wealthy nations, likely due to coevolutionary histories between STHs and human immune systems that favored anti-inflammatory pathways. Here, we document levels of intestinal inflammation and test associations with STH infection among the Shuar of Ecuador, an indigenous population undergoing socioeconomic/lifestyle changes that influence their hygienic environment. We predict that fecal calprotectin (FC; a measure of intestinal inflammation) will be lower in STH infected individuals and that FC will be negatively associated with infection intensity.Methods: Stool samples to analyze FC levels and STH infection were collected from 69 Shuar participants (ages 5-75 years). Children (<15 years) and adults (15+ years) were analyzed separately to understand the role of exposure in immune system development and the intestinal inflammatory response.Results: Two species of STH were present: Ascaris lumbricoides and Trichuris trichiura.The relationships between infection and intestinal inflammation were age-and species-specific. While no significant relationships were found among adults, children who were singly infected with T. trichiura had lower FC levels than uninfected children. Infection intensity was not significantly associated with FC in children or adults.Conclusions: These preliminary results provide limited support for our hypotheses, documenting tentative age-and species-specific associations between FC and infection status. Findings may point to the importance of species-specific STH exposure during immune system development. K E Y W O R D Sfecal calprotectin, hygiene hypothesis, inflammatory bowel disease, old friends hypothesis, soil-transmitted helminths

Background Soil-transmitted helminth (STH) infections have many negative health outcomes (e.g., diarrhea, nutritional deficiencies) that can also exacerbate poverty. These infections are generally highest among low-income populations, many of which are also undergoing market integration (MI; increased participation in a market-based economy). Yet the direct impact of MI-related social and environmental changes on STH infection patterns is poorly understood, making it unclear which lifestyle factors should be targeted to better control disease spread. This cross-sectional study examines if household infrastructure associated with greater MI is associated with lower STH burdens among Indigenous Ecuadorian Shuar. Methods Kato-Katz fecal smears were used to determine STH infection status and intensity (n = 620 participants; 308 females, 312 males, aged 6 months-86 years); Ascaris lumbricoides (ascarid) and Trichuris trichiura (whipworm) were the primary infection types detected. Structured interviews assessing lifestyle patterns (e.g., measures of household infrastructure) measured participant MI. Multilevel regression analyses and zero-inflated negative binomial regression models tested associations between MI measures and STH infection status or intensity, controlling for individual and community characteristics. Results Participants residing in more market-integrated households exhibited lower infection rates and intensities than those in less market integrated households. Parasite infection status and T. trichiura infection intensity were lower among participants living in houses with wood

Objective Anemia is an important global health challenge. We investigate anemia prevalence among Indigenous Shuar of Ecuador to expand our understanding of population‐level variation, and to test hypotheses about how anemia variation is related to age, sex, and market integration. Methods Hemoglobin levels were measured in a total sample of 1650 Shuar participants (ages 6 months to 86 years) from 46 communities between 2008 and 2017 to compare anemia prevalence across regions characterized by different levels of market integration. Results Shuar anemia rates among children under 15 years (12.2%), adult women (10.5%), and adult men (5.3%) were less than half of those previously documented in other neo‐tropical Indigenous populations. Anemia prevalence did not vary between more traditional and market integrated communities (OR = 0.47, p = .52). However, anemia was negatively associated with body mass index (OR = 0.47, p = .002). Conclusions Compared to other South American Indigenous populations, anemia prevalence is relatively low among Shuar of Ecuador and invariant with market integration. Understanding this pattern can provide valuable insights into anemia prevention among at‐risk populations.

This paper identifies the theme of honey gathering in Charlotte Brontë's fiction and places it within the context of Romantic and early Victorian representations of the nectarium's role in insect-flower relationships. Brontë's novels often invert the conventional use of botany to represent female sexuality by representing men as flowers and endowing her protagonists with an ulterior form of entomological agency. These insects work to express Brontë's desire for greater economic and erotic mobility, but it is argued that this mobility is problematized by the self-absorbed nature of the masculine nectarium, a dulcet gland in flowers originally believed by botanists to ooze sugar to serve a plant's own needs. This is particularly evident in Brontë's industrial romance Shirley (1849), as the theme of honey gathering is pathologized to visualize a crisis in the plotting of Victorian femininity. This paper may be helpful to scholars interested in Brontë's fiction, representations of sexuality, botany, entomology, ecology, and early Victorian pest discourse.

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