Microglia activation has emerged as a potential key factor in the pathogenesis of Alzheimer’s disease. Metabolite levels assessed by magnetic resonance spectroscopy (MRS) are used as markers of neuroinflammation in neurodegenerative diseases, but how they relate to microglial activation in health and chronic disease is incompletely understood. Using MRS, we monitored the brain metabolic response to lipopolysaccharides (LPS)-induced microglia activation in vivo in a transgenic mouse model of Alzheimer’s disease (APP/PS1) and healthy controls (wild-type (WT) littermates) over 4 hours. We assessed reactive gliosis by immunohistochemistry and correlated metabolic and histological measures. In WT mice, LPS induced a microglial phenotype consistent with activation, associated with a sustained increase in macromolecule and lipid levels (ML9). This effect was not seen in APP/PS1 mice, where LPS did not lead to a microglial response measured by histology, but induced a late increase in the putative inflammation marker myoinositol (mI) and metabolic changes in total creatine and taurine previously reported to be associated with amyloid load. We argue that ML9 and mI distinguish the response of WT and APP/PS1 mice to immune mediators. Lipid and macromolecule levels may represent a biomarker of activation of healthy microglia, while mI may not be a glial marker.
The objectives of this paper were to characterise lesions found at the hock and to establish their prevalence across a large sample of dairy cows in the UK. In addition, the study aimed to examine farmers' perceptions of these lesions. Seventy-seven farms were visited; both hocks on a random sample of approximately 50 cows were scored on a categorical scale for hair loss, ulceration and swelling. In addition, the size and location of lesions were marked on a hock map. A questionnaire designed to explore attitudes towards lesions was conducted with the owner. Hair loss and ulceration were seen at five locations. Hair loss was identified on 5,619 of 6,896 hocks (mild: 43.7 per cent; moderate: 25.1 per cent; severe: 12.6 per cent) and 1,137 of 6,896 hocks had areas of ulceration (mild: 8.6 per cent; moderate: 5.8 per cent; severe: 2.1 per cent). The majority of lesions were small; however, a small proportion had extensive areas of hair loss (maximum size 113.3 cm2) and ulceration (maximum size 95.5 cm2). Moderate swelling was recorded on 20 per cent of hocks, and 2.0 per cent had severe swelling. Interview results suggested that more than 90 per cent of participants were aware of hock lesions and a third had sought treatment for them; most felt that lesions had at least some impact on productivity and welfare.
A dimension reduction technique is proposed for matrix data, with applications to face recognition from images. In particular, we propose a factored covariance model for the data under study, estimate the parameters using maximum likelihood, and then carry out eigendecompositions of the estimated covariance matrix. We call the resulting method factored principal components analysis. We also develop a method for classification using a likelihood ratio criterion, which has previously been used for evaluating the strength of forensic evidence. The methodology is illustrated with applications in face recognition.
A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk with whole straw or rape straw bedding had a larger area of hair loss. 37Area of hair loss, as measured on hock maps, was not significantly different between 38 cows with score 1 (median=23.6 cm 2 ) and score 2 (median=20.3 cm 2 ) on the categorical scale for 39 hock lesions. This suggests that the categorical scale was not reflecting the extent of hair loss and 40 that hock maps are a good alternative for studying the dynamics of hock lesions over time. 41Further work is required to explore the aetiology of hock lesions and find better ways to control 42 this common condition.
Some methods for the statistical analysis of surface shapes and asymmetry are introduced. We focus on a case study where magnetic resonance images of the brain are available from groups of 30 schizophrenia patients and 38 controls, and we investigate large-scale brain surface shape differences. Key aspects of shape analysis are to remove nuisance transformations by registration and to identify which parts of one object correspond with the parts of another object. We introduce maximum likelihood and Bayesian methods for registering brain images and providing large-scale correspondences of the brain surfaces. Brain surface size-and-shape analysis is considered using random field theory, and also dimension reduction is carried out using principal and independent components analysis. Some small but significant differences are observed between the the patient and control groups. We then investigate a particular type of asymmetry called torque. Differences in asymmetry are observed between the control and patient groups, which add strength to other observations in the literature. Further investigations of the midline plane location in the 2 groups and the fitting of nonplanar curved midlines are also considered.
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