Prior to the Automated Liquids Processing System (ALPS) project, generation and maintenance of high-throughput (HTS) library and concentration-response sets was a labor intensive, batch-based process with multiple distinct operations. Because processes relied on a manual presence to move plates between workstations in separate laboratories, production was limited to core employee hours and humidity control was not feasible. Operations preceding plate-toplate transfers included but were not necessarily limited to centrifugation, unsealing/piercing, and labeling. Source plates were manually centrifuged to ensure liquids did not cling to seal material and to mitigate air bubbles prior to transfer. Individually, source compound plates were manually unsealed if in a 96/384 fixed-well plate format. Alternately, source 96/384 punch tube plates were pierced singly using a Remp Universal Plate Piercer (Brooks Automation, Inc., Chelmsford, MA). If pretransfer DMSO dilution was required, plates were manually loaded onto broadcast dispensers equipped with stacking capability (Zoom MV; Titertek Instruments, Huntsville, AL). Likewise, if destination plates were part of a dilution series set, DMSO was also dispensed using the Zoom MV. Destination plates, if not prelabeled with a barcode by the manufacturer, were manually stacked onto a CyBi-Print 458777J LAXXX10.
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