Tight regulation of cellular processes is key to the development of complex organisms but also vital for simpler ones. During evolution, different regulatory systems have emerged, among them RNA-based regulation that is carried out mainly by intramolecular and intermolecular RNA–RNA interactions. However, methods for the transcriptome-wide detection of these interactions were long unavailable. Recently, three publications described high-throughput methods to directly detect RNA duplexes in living cells. This promises to enable in-depth studies of RNA-based regulation and will narrow the gaps in our understanding of RNA structure and function. In this review, we highlight the benefits of these methods and their commonalities and differences and, in particular, point to methodological shortcomings that hamper their wider application. We conclude by presenting ideas for how to overcome these problems and commenting on the prospects we see in this area of research.
Structural Health Monitoring systems are developed to cost-efficiently prevent failure of mechanical and civil structures, and to predict the structure's residual life. In this work, a damage detection algorithm based on the Hilbert transform of the recorded signals from induced guided ultrasonic waves is presented. By means of this algorithm, damage localization in multi-wire cables is performed through a time-of-flight analysis of the wave packets. The algorithm is fully automated and distinguishes between wave packets from different waves independently. Its applicability is analyzed for laboratory experiments on a single cylindrical wire and on multi-wire cables. As an additional damage indicator, second harmonic waves are evaluated. Furthermore, the possibility to perform damage identification by evaluating the waves' amplitudes is analyzed. The amplitudes are compared with reference data from a novel hybrid finite-boundary element method.
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