Whole-cell, presynaptic calcium current (I Ca). By using an AP waveform as a voltage command, one can estimate Spacetime of fast presynaptic Ca 2+ signalling J Physiol 547.3 Glutamate release measured as excitatory postsynaptic current (EPSC). In voltage clamp, the EPSC in response to the presynaptic I Ca can be measured. Its time of onset (5 %
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