Process controls for mesophilic dark fermentation using a pH-dependent feeding strategy without NaOH addition were examined. One reactor utilizing microorganism holdback and two continuously stirred-tank reactors (CSTRs) with the same volume were employed. These were fed with pig manure and glucose as cosubstrates. Different hydraulic retention times were applied. The target pH was 5.5 within a limited range and controlled by varying organic loading rate. When the pH target interval was exceeded, the hydrogen production rate (HPR) decreased, and CH 4 was produced. A pH below the target interval had positive effects on the HPR.
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