Tests have been made to regenerate plants from different lupin species using leaf, leaf petiole and hypocotyl tissue of L. polyphyllus, L. hartwegii, L. angustifolius and L. luteus. Callus induction rates have been high with all plant parts from all tested lupin species. Regeneration success has been rather limited. One plant could be regenerated from hypocotyl material of L. polyphyllus. Only shoots appeared when using leaf explants from L. luteus or L. hartwegii as well as from leaf petiole tissue of L. hartwegii. With L. angustifolius one regeneration of unknown character occurred.
Transformed cell suspension and hairy root cultures were established by infecting seedlings of Lupinus polyphyllus and L. hartwegii with various wild type strains of Agrobacterium tume-faciens and A. rhizogenes. Transformation of the cultures was confirmed either by their phytohormone autotrophy, detection of opines or southern analysis. Glueosides of genistein and 2′-hydroxygenistein, were found to be the main secondary metabolites in normal and transformed suspension cultures as well as in hairy root cultures. Although some of the isoflavone glycosides of the cultures were apparently new constituents of Lupinus, they were afterwards also found in young seedlings.
The aim of this research work was to study the effect of variety and cultivation region on mineral elements and protein content of white asparagus spears (Asparagus officinalis L.). Therefore an extended field survey was conducted in the regions of Braunschweig (Lower Saxony) and Erfurt (Thuringia). Statistically significant differences between cultivation regions were found for Ca, Mg and sulphate. Additionally, spear samples of crop performance experiments with new varieties and genotypes in Lower Saxony and Schleswig-Holstein were analyzed. For nitrate and sulphate significant differences were found between varieties in Schleswig-Holstein. In Lower Saxony, genotypical differences were determined for nitrate, sulphate, Ca, S, Fe, Zn, Mn and Cu.
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