Prion diseases are neurodegenerative infectious disorders for which no prophylactic regimens are known. In order to induce antibodies/auto-antibodies directed against surface-located PrP c , we used a covalently linked dimer of mouse prion protein expressed recombinantly in Escherichia coli. Employing dimeric PrP as an immunogen we were able to effectively overcome autotolerance against murine PrP in PrP wild-type mice without inducing obvious side effects. Treatment of prion-infected mouse cells with polyclonal anti-PrP antibodies generated in rabbit or auto-antibodies produced in mice significantly inhibited endogenous PrP Sc synthesis. We show that polyclonal antibodies are binding to surface-located PrP c , thereby interfering with prion biogenesis. This effect is much more pronounced in the presence of full IgG molecules, which, unlike Fab fragments, seem to induce a significant cross-linking of surface PrP. In addition, we found immune responses against different epitopes when comparing antibodies induced in rabbits and PrP wild-type mice. Only in the auto-antibody situation in mice an immune reaction against a region of PrP is found that was reported to be involved in the PrP Sc conversion process. Our data point to the possibility of developing means for an active immunoprophylaxis against prion diseases.

Purpose-Various types and implementations of crowdsourcing have emerged on the market; many of them are related to logistics. While we can identify plenty of crowd logistics applications using information technology capabilities and information sharing in practice, theories behind this phenomenon have received only limited attention. This paper accounts for filling this research gap by analyzing the crowd's contributions in logistics of goods and information. Thereby, this paper aims to provide the necessary basis for a novel interdisciplinary research field. Design and Methodology-This paper is part of an ongoing research endeavor in the field of location-based crowdsourcing (LBCS). It represents conceptual work that builds on a literature review enriched with an in-depth analysis of real-world examples in the field of crowd logistics. Using a scoring method, we provide an example how a company may evaluate the alternatives of crowd logistics. Approach-The main approach is an analysis of variants of how the social crowd may be integrated in logistics processes. The work is conceptual in its core. Thereby, we use real-world examples of crowdsourcing applications to underpin the evaluated variants of crowd logistics. Findings-The paper presents relevant theoretical background on crowd logistics. We differentiate between variants of crowd logistics with their flow of materials, goods, and information. Thereby we zoom in the type, significance, and process flow of the crowd's contributions. We discuss potential advantages and challenges of logistics with the performing crowd and deeply discuss opportunities and challenges from a business and from an individual's perspective. Finally, we highlight a route map for future research directions in this novel interdisciplinary research field. Limitations of the research-As this work is conceptual in its core, generalizations may be drawn only with great care. Still, we are in a position to propose a route map for further research in this area in this paper. Also the integration of an analysis of a scale of real-world applications allows us to highlight our research's practical relevance and implications. Contributions-The main contribution of this paper is an in-depth analysis and consolidation of innovative crowd logistics applications in order to provide an overview on recent implementations. We propose a categorization scheme and contribute with a route map for further research in the field of crowd logistics.

Hereditary neuropathies comprise a wide variety of chronic diseases associated to more than 80 genes identified to date. We herein examined 612 index patients with either a Charcot-Marie-Tooth phenotype, hereditary sensory neuropathy, familial amyloid neuropathy, or small fiber neuropathy using a customized multigene panel based on the next generation sequencing technique. In 121 cases (19.8%), we identified at least one putative pathogenic mutation. Of these, 54.4% showed an autosomal dominant, 33.9% an autosomal recessive, and 11.6% an X-linked inheritance. The most frequently affected genes were PMP22 (16.4%), GJB1 (10.7%), MPZ, and SH3TC2 (both 9.9%), and MFN2 (8.3%). We further detected likely or known pathogenic variants in HINT1, HSPB1, NEFL, PRX, IGHMBP2, NDRG1, TTR, EGR2, FIG4, GDAP1, LMNA, LRSAM1, POLG, TRPV4, AARS, BIC2, DHTKD1, FGD4, HK1, INF2, KIF5A, PDK3, REEP1, SBF1, SBF2, SCN9A, and SPTLC2 with a declining frequency. Thirty-four novel variants were considered likely pathogenic not having previously been described in association with any disorder in the literature. In one patient, two homozygous mutations in HK1 were detected in the multigene panel, but not by whole exome sequencing. A novel missense mutation in KIF5A was considered pathogenic because of the highly compatible phenotype. In one patient, the plasma sphingolipid profile could functionally prove the pathogenicity of a mutation in SPTLC2. One pathogenic mutation in MPZ was identified after being previously missed by Sanger sequencing. We conclude that panel based next generation sequencing is a useful, time- and cost-effective approach to assist clinicians in identifying the correct diagnosis and enable causative treatment considerations.

Previously, we reported gene amplification at chromosome 3q26-27 in more than one third of squamous cell carcinomas of the lung. Frequent amplification of eukaryotic translation initiation factor 4G on 3q27.1 indicated a possible role of this amplification in translation initiation. The analysis of 61 squamous cell lung carcinomas shows that the percentage of carcinomas with a 3q27.1 amplification increases in higher malignant tumors. Non-invasive (T1) and minimal-invasive (T2) tumor stages showed similar percentages of amplified and non-amplified tumors, whereas locallyinvasive (T3) tumors revealed a statistically significant (p < 0.05) increased percentage of amplified tumors. Microarrays were used to analyze the expression pattern of genes mapping in the amplified domain and its flanking regions (3q25-28) as well as the expression of genes directly or indirectly associated with translation initiation in squamous cell carcinoma, large cell carcinoma, adenocarcinoma and small cell carcinoma. Three genes, namely FXR1, CLAPM1 and EIF4G, are most frequently overexpressed in the center of the amplified domain in squamous cell carcinomas. The eukaryotic translation initiation factors 4A1, 2B and 4B as well as the poly(A)-binding protein PABPC1 where found to be overexpressed in all lung cancer entities. We found, however, no overexpression of eIF4E. Our results contribute to the understanding of the frequent amplification processes in squamous cell carcinomas of the lung and to the understanding of the translation initiation that appears not to require eIF4E in lung carcinogenesis. ' 2007 Wiley-Liss, Inc.