Lymphocystis virus has been implicated as the cause of severe infection, mortality, and economic loss in farmed giant snakehead (Channa striata) or gabus fish in Mandiangin South Kalimantan. In Kalimantan, the fish is locally known as Haruan fish and considered to have health-related benefits to human due to its high albumin content. This study aimed to determine the LCDV in gabus fish through histopathological and PCR investigation. Infected LCDV fish have a cluster of warts growths in the skin or fin of a walleye. Infected fishes with an average of total length of 15 cm and weight of 150 g were collected from local net cages and earthen ponds. They were transferred into a laboratory, anesthetized, and then subsequently killed by organ dissection. The infected fish tumors were aseptically cut out for histological study and PCR detection. Primer used was forward GII F: 5 ‘TGG GAT TCC AAY GGT CAA TTA-3’ with target band of 468 bp (for genotype-IIi LCDV) and primer reverse R: 5’TTA GAT TAT TGG GCA GCG TT-3' with target band of 250 bp (for genotype-II LCDV) and GIII F: 5' AGG AAA TAA CCG CAG TA GAA TGCA. Lymphocytosis in infected fish showed multifocal to diffuse white, round, firm, papilloma or tumor-like nodules on the skin of the body, fins, eyes, and mouth. The hypertrophied cell was surrounded by a thick smooth hyaline capsule. Stress condition caused by high population density, nutrition deficiencies, decreased dissolved oxygen, suboptimal water quality, and handling may increase the appearance of LCDV symptoms. The result of the present study revealed that histopathology and PCR could be used to diagnose LCDV infection.
Penyakit tumor mutiara (pearl-like nodul disease) yang disebabkan oleh virus dari genus Lymphocyctis, Family Iridoviridae merupakan salah satu kendala pada budidaya ikan gabus. Tujuan penulisan ini adalah mendiskripsikan penyakit tumor mutiara (LCDV) pada ikan gabus dari identifikasi virus penyebabnya, cara deteksi dan pengendalian LCDV di akuakultur. Kondisi stress merupakan pemicu berkembangnya penyakit LCDV atau Lymphocystis Disease Virus ini pada tubuh ikan. Virus yang bersifat dermotropic ini menyebabkan penyakit yang bersifat self-limiting disease, atau dapat sembuh dengan sendirinya jika kondisi tubuh dan lingkungannya membaik. Deteksi level 1 seperti gejala klinis, level 2 melalui histopatologis dan level 3 yang bersifat konfirmasi dengan PCR, in situ hybridisasi maupun loop mediated isothermal amplification untuk skrining infeksi yang bersifat subklinis telah banyak digunakan. Gejala klinis yang khas adalah timbulnya tumor menyerupai buah anggur atau mutiara. Histopatologi yang pathognomonis adalah sel limfosit hipertrofi dikelilingi kapsul hyaline dan ditemukan basophilic intracytoplasmic inclusion. PCR konvensional dan qPCR digunakan untuk deteksi kualitatif dan kuantitatif. Sinyal hibridisasi pada deteksi ISH menunjukkan adanya perkembangan virus di dalam tubuh ikan. Ikan dapat sembuh dengan sendirinya dan mempunyai kekebalan perolehan. Kondisi ikan yang sehat dan lingkungan yang baik dapat mencegah berkembangnya virus.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.