This study aimed to evaluate the physiological variations in the serum biochemical profile of neonate (3-5 days old) Senepol and crossbreed Holstein x Gir calves in Uberlândia, state of Minas Gerais, Brazil, and to compare values between breeds and sexes. In total, 168 healthy Senepol (n = 80, 28 females and 52 males) and crossbred Holstein x Gir (n = 88, 49 females and 39 males) calves were evaluated. From each animal, approximately 5 mL of blood was collected by jugular venipuncture into a sterile tube without anticoagulant to obtain serum. Serum concentrations of total protein, albumin, creatinine, urea, total cholesterol, triglycerides, calcium, and phosphorus; and the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, and gamma-glutamyl transferase (GGT) were determined on a automated multichannel analyzer. There were significant differences (p < 0.05) in serum concentrations for total protein, globulin, albumin/globulin ratio (A:G), creatinine, urea, phosphorus, calcium/phosphorus ratio (Ca:P), AST, and GGT between Senepol calves and crossbred Holstein x Gir calves, but no significant differences in serum concentrations were observed between male and female calves for either breed (p > 0.05). It was concluded that the serum biochemistry profile of newborn Senepol and crossbred Holstein x Gir calves was affected by differences in colostrum intake management and breed characteristics. Also, no differences in blood parameters were observed between male and female calves.
Agradeço primeiro a Deus, Jesus e toda espiritualidade que me assiste, sem a qual nem aqui hoje eu estaria. Aos meus pais, Rozélia e Manoel, por me aceitarem como filha, me apoiarem desde a infância a realizar meu sonho de ser "médica de bichos" e em toda a minha trajetória nessa profissão que amo. Aos meus irmãos, Henrique e Romeu, que são meus melhores amigos, sempre com palavras encorajadoras e conversas descontraídas, não me deixando desistir nunca! Ao meu marido, companheiro de vida, pai das minhas filhas, João Gabriel, que atura meu estresse diário, cuida de mim e me ama (mesmo assim). Às minhas flores, Manuela, Gabriela e Marina, que são lenha na fogueira da minha vida, meus maiores projetos e desafios, amo vocês mais do que a mim mesma. Aos meus sogros, Mariana e João Leopoldo, por toda dedicação com as minhas filhas, e amizade permitindo assim que eu tivesse mais tempo para IX ABSTRACT Despite being considered a bacteria that is part of the natural microbiota of dogs' skin, S. pseudintermedius has been associated with infections in skin and delicate tissues in dogs, as well as some cases of infections in humans. The thesis was divided into three chapters, the first referring to the general considerations of topics covered in the other chapters. The second chapter aimed to evaluate the resistance profile to a panel of more than 30 antimicrobials, with bacteria isolated from 698 clinical samples of dogs between the years 2016 and 2018. For the evaluation, results from diffusion tests on discs performed in a private laboratory between the years 2016 to 2018 were used. The analysis of the results determined the etiological agents mostly involved in infections and the temporal evolution of antibacterial resistance. The third chapter aimed to evaluate the presence of S. pseudintermedius in clinical samples taken from dogs' skins and ears, and the nostrils of their guardians. As well as evaluate the presence of resistance genes mecA, mecA1 and mecA2, which confer resistance to β-lactam antimicrobials, gene msr responsible for resistance to macrolides, and virulence genes FnbB (cell invasion) and PVL (leukotoxin), using PCR technique. The in vitro performance of antimicrobials oxacillin, cefovecin and gentamicin was determined by the methods of disk diffusion and minimum inhibitory concentration-MIC. It also determined the association of factors related to the animal's lifestyle with infection with S. pseudintermedius, an association between the presence of genes and antimicrobial resistance and the tests.
The aim of this study was to evaluate the blood profile and haematological changes in obese adult and senior dogs. An evaluation was made from the blood profile of 59 obese adult and senior dogs. The animals were divided into two groups, group 1 (G1) with 30 obese adult dogs (2 to 8 years) and group 2 (G2) with 29 obese senior dogs (over 8 years old). The mean values of the erythrogram and platelet count parameters of both groups remained within the compared reference ranges except the red cell distribution width (RDW), whose values were lower than the reference ranges in both groups. A comparison of the values of erythrogram and platelet count of the groups indicated that the RDW and MPV (mean platelet volume) of G2 were higher than those of G1. As for the white blood cell count (WBC), only the number of band neutrophils exceeded the physiological limits for the species in both groups, with no significant difference in values between the age groups. The males in G2 showed a significantly higher mean eosinophil count than those in G1 and than the females of both groups. As for the frequency of findings, 90% of the animals in G1 and 68.96% in G2 showed left shift neutrophils (LSN), while 34.48% in G1 and 20% in G2 showed eosinophilia. It was concluded that, irrespective of age, obese animals presented no changes in erythrogram and platelet indices, and that their WBC may exhibit discrete LSN without leukocytosis. Complete blood count, white blood cell count, obesity
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