Here we demonstrate that partially purified Xenopus p42 mitogen-activated protein (MAP) kinase phosphorylates bacterially expressed human c-Jun at a single site, serine 243. Several lines of evidence argue that this phosphorylation is due to p42 MAP kinase itself rather than some contaminating species. Phosphorylation of serine 243 markedly decreases the binding of c-Jun to oligonucleotides containing the 12-O-tetradecanoylphorbol-13-acetate response element. These findings suggest that MAP kinase may play a role in the down-regulation of c-Jun or in the cycle of transcriptional initiation and elongation.
Currently additive manufacturing (AM) is a rapidly developing industrial sector and a disruptive technology. It is an answer for new challenges such as resources saving and energy effectiveness as well as response for circular economy needs. Unfortunately, the full exploitation of 3D printing technology for ceramic is currently limited due to the in-process and in-service performance of the available materials’ sets, especially in application in construction industry. The main aim of the article is comparison mechanical properties such as compressive and flexural strength between casted and injected samples (simulation for 3D printing process). The same geopolymer mix, plain as well as with flax fibres, based of fly ash class F was casted and cured in the same temperature prior to its mechanical test with ‘printed’ samples.
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