The relationship between different mechanoregulatory factors and mechanisms by which neuronal development is regulated by diverse biophysical cues in the immediate vicinity of neural cells remains sparsely understood. Considering the extreme physical complexity of neuronal niches, it is imperative to look beyond the chemical cues for a holistic understanding in these lines. Here the role of geometrically diverse physical microcues is studied in morphologically regulating neurite branching, direction, growth, and network formation in nascent primary cortical neurons on a simple cytocompatible interface. The results indicate distinct differences in neurite branching, microgap sensing, and path‐finding tendencies in response to the underlying angled/curved cues on the micropatterned interface. While the microtraps along curved cues promote higher branching, the highly angled cues promote relatively lower branching with higher neurite lengths. In this study specific morphological changes induced by these cues on the collective conformity, branching, and mean length of neurites in both isolated neurons and neurons in networks are investigated. Finally, a model is proposed to study mechanoregulation of isolated neurons and neuronal networks in response to diverse physical cues. Overall, this platform aims to propel efficient morphological modulation for the study of neurodevelopmental anomalies or physical guidance inspired repair strategies.
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