High-throughput live-imaging of embryos is an essential technique in developmental biology, but it is difficult and costly to mount and image embryos in consistent conditions. Here, we present OMMAwell, a simple, reusable device to easily mount dozens of embryos in arrays of agarose microwells with customizable dimensions and spacing. OMMAwell can be configured to mount specimens for upright or inverted microscopes, and includes a reservoir to hold live-imaging medium to maintain constant moisture and osmolarity of specimens during time-lapse imaging. All device components can be fabricated by cutting pieces from a sheet of acrylic using a laser cutter or by making them with a 3D printer. We demonstrate how to design a custom mold and use it to live-image dozens of embryos at a time. We include descriptions, schematics, and design files for 13 additional molds for nine animal species, including most major traditional laboratory models and a number of emerging model systems. Finally, we provide instructions for researchers to customize OMMAwell inserts for embryos or tissues not described herein.
The purpose of this study was to provide an analysis of an international sporting event as a possible catalyst for social change. Because of the unique circumstances surrounding the bid process, the dual hosting of the World Cup 2002 by Korea and Japan was seen as a unique opportunity to examine the power of sport as a catalyst behind change. Longitudinal secondary data were consulted to look at the economic, social and cultural impact of the event, while interviews with respondents in both nations gave more insight on how the respondents viewed the relationship between the two nations. Economic, social and cultural indicators all reflected an impact of the World Cup on the bilateral relationship. The interviews suggested that there were two main barriers to an improved relationship between the two nations (Victim mentality of the Korean toward the Japanese, Lack of awareness of Korea in Japan), and that it was not necessarily the organization of the event that alleviated these barriers, but the performance of the Korean football team.
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