The biosynthesis of 2'-deoxymugineic acid, a key phytosiderophore, was examined in association with the putative methionine recycling pathway in the roots of wheat using labeling experiments and structural analysis. Feeding with D-[1-13C]ribose did not result in 13C enrichment of 2'-deoxymugineic acid, while D-[2-13C]ribose resulted in 13C enrichment at the C-4", -1, -4' positions, and D-[5-13C]ribose did in C-1', -4, and -1" positions of 2'-deoxymugineic acid, respectively. Furthermore, two isotope-labeled intermediates of the methionine recycling pathway, 5-[5-2H2]methylthioribose and 2-[1-13C]keto-4-methylthiobutyric acid, were synthesized, and their incorporation into 2'-deoxymugineic acids was investigated. Six deuterium atoms at the C-4, -1', and -1" positions of 2'-deoxymugineic acid were observed after feeding with 5-[5-2H2]methylthioribose. Feeding with 2-[1-13C]keto-4-methylthiobutyric acid yielded 2'-deoxymugineic acid enriched with 13C at the C-4', -1, and -4" positions. These results demonstrated for the first time that the biosynthesis of 2'-deoxymugineic acid is associated with the methionine recycling pathway. This association system functions to recycle methionine required for continued synthesis of mugineic acids in the roots of gramineous plants.
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.