The objective of the study was to investigate the potential application of beta-glucans obtained from yeast sediment resulting from the manufacture of local Viorica wine (2018 harvest). To determine the amount of beta-glucans in the yeast sediment, two were used: the calculation method and the laboratory method, obtaining similar quantities, 29.92 ± 0.47 and 28.17 ± 0.32 respectively. The beta -glucans obtained were incorporated in various concentrations (0.1%; 0.2%; 0.3%; 0.4% and 0.5%) in the yogurt obtained from skimmed milk. The effect of beta-glucan addition on the physicochemical properties of freshly prepared yogurts was investigated. The addition of beta-glucans positively influenced the formation of the gel relay resulting in a decrease in the fermentation time of yogurt. The final pH point of 4.5 was reached one hour earlier (in 4 hours) compared to the control sample (in 5 hours). The results showed that there are no significant changes in physicochemical properties (titratable acidity, pH, viscosity and syneresis). The results obtained report that beta-glucans can be used as a thickening agent for low-fat yogurts by shortening the fermentation period and not essentially changing the sensory characteristics. Experimental results showed that the glycemic index of yogurt samples with the addition of beta-glucans have similar values in the range of 28-30. Respectively, the yogurts under study are attributed to food category with low glycemic index.
This paper reports about experimental results concerning the influence of 30 nm ZnO nanoparticles on biomass, carbohydrates, β-glucans, proteins accumulation and catalase enzyme activity at Saccharomyces cerevisiae CNMN-Y-20 yeast strain exposed to alcohol action. Alcohol in concentrations of 2%, 5% and 10% added to culture medium has been reported to stimulate β-glucans biosynthesis and to inhibit protein synthesis. Low biomass production, with 71% less that control, was detected in the experiments with 10% alcohol. ZnO nanoparticles in combination with alcohol do not offer sufficient protection for the proteins biosynthesis, but efficiently protect the carbohydrates and β-glucans biosynthetic processes, which contents in the biomass are with 16.6% and 19.9% higher than control, respectively. The maximum value of β-glucans content was established in case of cultivation of selected yeast strain on YPD medium supplemented with 5 mg/L nanoparticles ZnO and 2% alcohol. The obtained results allowed the elaboration of new procedure for directed synthesis of β -glucans that contributed to an increase of this component with 30.7%, compared to control.
Currently, special attention is paid to the use of industrial by-products, in particular those obtained in huge quantities in the brewing and wine making process, as a source for the production of natural preparations with high biological value. In this study are presented results related to the recovery of beer yeast biomass from the sediments of the beer industry, by obtaining lipid extracts with valuable biochemical composition. Thus, applying the optimized autolysis method, with the use of sodium phosphate buffer at 45°C for the destruction of the cell wall and fractional extraction with hydric, alkaline and acidic solutions, various liquid and solid fractions were obtained from the biomass of brewer’s yeast with varied lipid content. So, in order to optimize the waste processing process of the beer industry and the complex recovery of yeast biomass, it is reasonable to include the lipid fraction extraction stage in the technological flow, after obtaining protein and mannoprotein extracts, which will be useful and as an additional step of purification of the β-glucan fraction.
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