Objective-We tested the hypothesis that direct native low-density lipoprotein (LDL) injection into LDL receptordeficient (LDLR Ϫ/Ϫ ) mice would induce the adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in their aortic endothelial cells, and that transcriptional regulation of this pathway involved activator protein-1 (AP-1) but not nuclear factor B (NF-B).
Methods and Results-Using tail vein injection of LDL into LDLRϪ/Ϫ mice, we were able to maintain atherogenic LDL blood levels, which induced ICAM-1 and VCAM-1 expression in their aortic endothelial cells after 24 hours. We were able to visualize and quantify this expression using immunohistochemistry and confocal microscopy. Under conditions in which ICAM-1 and VCAM-1 were expressed, the regulatory AP-1 proteins c-Fos and c-Jun were also highly expressed in the endothelial cell cytoplasm and observed within the cell nucleus. The NF-B protein P65, although expressed in the endothelial cell cytoplasm after LDL injection, was not observed within the cell nucleus.
Conclusions-Elevated
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