No abstract

Summary1. Boreal ecosystems are warming roughly twice as fast as the global average, resulting in woody expansion that could further speed up the climate warming. Boreal peatbogs are waterlogged systems that store more than 30% of the global soil carbon. Facilitative effects of shrubs and trees on the establishment of new individuals could increase tree cover with profound consequences for the structure and functioning of boreal peatbogs, carbon sequestration and climate. 2. We conducted two field experiments in boreal peatbogs to assess the mechanisms that explain tree seedling recruitment and to estimate the strength of positive feedbacks between shrubs and trees. We planted seeds and seedlings of Pinus sylvestris in microsites with contrasting water-tables and woody cover and manipulated both shrub canopy and root competition. We monitored seedling emergence, growth and survival for up to four growing seasons and assessed how seedling responses related to abiotic and biotic conditions. 3. We found that tree recruitment is more successful in drier topographical microsites with deeper water-tables. On these hummocks, shrubs have both positive and negative effects on tree seedling establishment. Shrub cover improved tree seedling condition, growth and survival during the warmest growing season. In turn, higher tree basal area correlates positively with soil nutrient availability, shrub biomass and abundance of tree juveniles. 4. Synthesis. Our results suggest that shrubs facilitate tree colonization of peatbogs which further increases shrub growth. These facilitative effects seem to be stronger under warmer conditions suggesting that a higher frequency of warmer and dry summers may lead to stronger positive interactions between shrubs and trees that could eventually facilitate a shift from moss to tree-dominated systems.

No abstract

Similar to humans, the fecal microbiome of dogs may be useful in diagnosing diseases or assessing dietary interventions. the accuracy and reproducibility of microbiome data depend on sample integrity, which can be affected by storage methods. Here, we evaluated the ability of a stabilization device to preserve canine fecal samples under various storage conditions simulating shipping in hot or cold climates. Microbiota data from unstabilized samples stored at room temperature (Rt) and samples placed in PERFORMAbiome•GUT collection devices (PB-200) (DNA Genotek, Inc. Ottawa, Canada) and stored at RT, 37 °C, 50 °C, or undergoing repeated freeze-thaw cycles, were compared with freshly extracted samples. Alpha-and beta diversity indices were not affected in stabilized samples, regardless of storage temperature. Unstabilized samples stored at Rt, however, had higher alpha diversity. Moreover, the relative abundance of dominant bacterial phyla (firmicutes, Fusobacteria, Bacteriodetes, and Actinobacteria) and 24 genera were altered in unstabilized samples stored at RT, while microbiota abundance was not significantly changed in stabilized samples stored at Rt. our results suggest that storage method is important in microbiota studies and that the stabilization device may be useful in maintaining microbial profile integrity, especially for samples collected off-site and/or those undergoing temperature changes during shipment or storage.

No abstract

No abstract

Whole-plant corn silages treated with either Pioneer 1174® or Biotal® inoculants were preserved more efficiently than control silages. They had slightly higher dry matter (DM) recoveries; more lactic acid; higher lactic to acetic acid ratios; and less acetic acid, ethanol, and ammonia-nitrogen. Laboratory silo results showed that both inoculated silages produced lactic acid faster than control silages during the first 7 days and had more desirable fermentation profiles at the end of 90 days. Applying 5 or 10 times the recommended rate of Biotal inoculant had only a small and nonsignificant effect on rate and efficiency of fermentation. Yearling steers fed the two inoculated corn silages gained numerically but not significantly faster and more efficiently than steers fed control silages, so inoculated silages produced about 6.1 lb more steer gain per ton of crop ensiled than controls. SummaryWhole-plant corn silages treated with either Pioneer 1174® or Biotal® inoculants were preserved more efficiently than control silages. They had slightly higher dry matter (DM) recoveries; more lactic acid; higher lactic to acetic acid ratios; and less acetic acid, ethanol, and ammonia-nitrogen. Laboratory silo results showed that both inoculated silages produced lactic acid faster than control silages during the first 7 days and had more desirable fermentation profiles at the end of 90 days. Applying 5 or 10 times the recommended rate of Biotal inoculant had only a small and nonsignificant effect on rate and efficiency of fermentation.Yearling steers fed the two inoculated corn silages gained numerically but not significantly faster and more efficiently than steers fed control silages, so inoculated silages produced about 6.1 lb more steer gain per ton of crop ensiled than controls.

The effect of 1174® Silage Inoculant on the ensiling process was studied using three Pioneer corn hybrids. All hybrids fermented rapidly, and 1174 did not significantly influence any of the fermentation characteristics during the 120 days. The epiphytic lactic acid bacteria (LAB) counts on the chopped corn plants were high; 14 times greater than the numbers of LAB provided by the inoculant. Although during fermentation, statistically significant differences occurred among the hybrids for fermentation endproducts, no observed trends suggested that hybrid effects were real.