Using sensitive assays, the prevalence of gestational thyrotoxicosis in Asian women was found to be 11.0% and was significantly higher in subjects at 8-11 weeks of gestation than at 12-14 weeks. The positive correlation between hCG and free T4 seen in patients tested at 8-11 weeks was absent in patients tested at later stages of the first trimester. Future studies investigating the entity of gestational thyrotoxicosis, at least in Asian patients, should focus on patients at earlier stages of gestation than currently practised.

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Clot activator serum tubes have significantly improved turnaround times for result reporting compared to plain tubes. With increasing workload and service performance expectations confronting clinical laboratories with high-volume testing and with particular emphasis on critical analytes, attention has focussed on preanalytical variables that can be improved. We carried out a field study on the test performance of BD vacutainer rapid serum tubes (RSTs) compared to current institutional issued BD vacutainer serum separator tubes (SSTs) in its test result comparability, clotting time, and stability on serum storage. Data from the study population (n = 160) of patients attending outpatient clinics and healthy subjects showed that results for renal, liver, lipids, cardiac, thyroid, and prostate biochemical markers were comparable between RSTs and SSTs. Clotting times of the RSTs were verified to be quick with a median time of 2.05 min. Analyte stability on serum storage at 4°C showed no statistically significant deterioration except for bicarbonate, electrolytes, and albumin over a period of 4 days. In conclusion, RSTs offered savings in the time required for the clotting process of serum specimens. This should translate to further trimming of the whole process from blood collection to result reporting without too much sacrifice on test accuracy and performance compared to the current widely used SSTs in most clinical laboratories.

Context: Point-of-care testing (POCT) for blood gas and chemistries is widely adopted in the emergency department (ED) for fast turnaround and critical medical decisions.Objective: To assess performance of ED physicians compared to laboratory technologists carrying out i-STAT analysis. Impact of user skill on POCT performance was also studied.Design: Over a 3-month period, ED physicians performed tests with their i-STAT unit with parallel blood specimens (n=60) sent to the central laboratory to be tested on another i-STAT unit and laboratory analysers (blood gas and chemistries). Comparisons to laboratory results (obtained with ABL 520 and Beckman-Coulter LX20PRO) were carried out. Two operators from ED and central laboratory performed side by side, repeated tests on the i-STAT units. Their overall within-run imprecision were compared. The analytical performance of i-STAT was further assessed by another laboratory technologist to verify its capability to deliver good results. Results:Platform comparisons showed that ED physicians performed poorer compared to laboratory technologists, particularly with chloride and pO2 (Spearman coefficient of correlation -0.49 and 0.54 respectively indicate wide scatter). This is also mirrored in the higher overall within-run imprecision for chemistries (sodium, chloride, potassium, glucose, urea and creatinine) by an ED physician (5.4%) compared to a laboratory technologist (3.7%). Including blood gas results showed an even wider gap in their testing skills. Except at a low creatinine of 67 μmol/L, the i-STAT analyser delivered precise measurements for the chemistries and blood gas parameters. Conclusions:Performances by medical staff with the i-STAT analyser did not adequately approach the same skill level as laboratory technologists.

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Objective: Various parameters had been used to predict ovarian response. Among them, Anti-Müllerian Hormone (AMH) and antral follicle count (AFC) demonstrate the most favourable analytical and performance characteristics. In this pilot study, we aim to determine the cut-off levels of AMH using automated AMH assays and AFC in the prediction of poor and high responders. Study Design: Prospective study of 43 women between 21 to 45 years old scheduled for assisted reproduction. AMH levels on day 3 of menstruation were analysed using two immunoassay kits, namely the Beckman Coulter Access AMH and the Roche Elecsys AMH on the two automated analysers Beckman Coulter DxI 800 and Roche Cobas e602 respectively. AFC was also assessed on day 3 of menstruation prior to in vitro fertilization (IVF). These were compared with the number of oocytes retrieved after controlled ovarian stimulation. Results: AMH (Beckman Coulter Access AMH and Roche Elecsys AMH) highly correlated with AFC and the number of oocytes retrieved after ovarian stimulation. Beckman Coulter Access AMH was the better predictor for poor ovarian response with ROC [Formula: see text] of 0.83. For the prediction of a high response, AFC had a higher ROC [Formula: see text] of 0.95. Through ROC, the AMH cut-off level for poor ovarian response was 2.23 ng/ml with Beckman Coulter Access AMH and 2.02 ng/ml with Roche Elecsys AMH, while the AMH cut-off for a high ovarian response was 5.19 ng/ml with Beckman Coulter Access AMH and 4.60 ng/ml with Roche Elecsys AMH. For AFC, the cut-off for poor ovarian response was 18 and for high response was 34. Conclusion: AMH and AFC are reliable predictors of ovarian response. Establishment of specific levels may improve individualised controlled ovarian stimulation and optimise the oocyte yield. Larger studies are required to establish these findings.