Iguanas are exotic animals that are widely kept as pets in our society,their performance and color being their main attraction. Jogja Exotarium is anin situ conservation park that carries the back to nature concept. Animals thatare kept at the Jogja Exotarium are allowed direct contact with visitors. Thisstudy aims to determine the hormone triiodothyronine in female iguanas that are raised and conserved in situ. This study used feces obtained from iguanas kept in Jogja Exotarium, sampling was carried out on Wednesdays and Sundays for 2 weeks. The collected faecal samples were processed by usingfreeze-drying method and then extracted using 80% methanol. The extracted samples were analyzed using the enzyme immunosorbent assay (EIA) method. The results showed during the week day was 107.1 ± 7.01 ng/gr dry feces, while the week end was 119.95 ± 4.3 ng/gr dry feces. Based on the results of the study, it can be concluded that there is no difference in the hormone triiodothyronine in female iguanas during weekday and weekend
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