In this study, a continuous cell imaging system with subcellular resolution by integrating a microfluidic platform with lattice lightsheet microscopy (LLSM) was developed. To reduce aberrations of the lightsheet propagating...
Abstract:Visible femtosecond laser is shown to be capable of selectively inactivating a wide spectrum of microorganisms in a wavelength and pulse width dependent manner.However, the mechanism of how visible femtosecond laser affects the viability of different microorganisms is still elusive. In this report, the cellular surface properties, membrane integrity and metabolic rate of Escherichia coli (E.coli) irradiated by a visible femtosecond laser (λ=415nm, pulse width=100fs) with different exposure time were investigated. Our results showed that femtosecond laser treatment for 60 minutes (min) led to cytoplasmic leakage, protein aggregation and alternation of the physical properties of E. coli cell membrane. In comparison, a 10 min exposure of bacteriatofemtosecond laser irradiation induced an immediate reduction of 75% of the glucose-dependent respiratory rate, while the cytoplasmic leakage was not detected.Results from enzymatic assays showed that oxidases and dehydrogenases involving in E.coli respiratory chain exhibited divergent susceptibility after laser irradiation. This early commencement of respiratory inhibition after a short irradiation is presumed to play a dominant effect on the early stage of bacteria inactivation.
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