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The present study compares health-related QOL between patients who practiced self-care activities comprising exercise and diet at home for at least 6 months (SA group; n = 84) after elective PCI and those who did not (NA group; n = 54). Health-related (HR) QOL was measured using the SF-36 questionnaire. Scores for two mental and three physical parameters of the HRQOL were increased in the SA, but not in the NA group. These findings indicated that the QOL was far superior among patients who exercised and consumed an appropriate diet at home for at least 6 months after elective PCI compared with those who did not.

Reduction of cellular damage in densely cultured cell monolayers after cryopreservation by pre-incubation with hyaluronan (HA) was investigated. Monolayers of human dermal fibroblasts were cultured for 24 h at a density of 0.5 × 10 4 or 5 × 10 4 cells/cm 2 . The following two experimental conditions were compared: cells incubated with or without 0.5% w/w HA solution for 6 h. Samples were frozen from 4 to −80 • C at 0.3 or 3 • C/min in a cryoprotectant solution containing 10% w/w dimethyl sulfoxide, cooled down below −185 • C, and then thawed. Post-thaw cell viability was evaluated by the fluorescent double-staining technique using a fluorescence microscope, and cellular uptake of the fluorescein-isothiocyanate-labeled HA after pre-incubation was also observed. Cell viability decreased with increasing cell density at both cooling rates without preliminary HA incubation. However, cell viability did not decrease at either cooling rate with preliminary HA incubation. Cellular HA uptake was observed. Pre-incubation with HA reduces cellular damage in densely cultured cell monolayers.

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